|
|
| Line 8: |
Line 8: |
| | ==[[/LABView]]== | | ==[[/LABView]]== |
| | | | |
| - | ==MT== | + | ==[/MT]== |
| - | *making a new batch of MT's w/ Igor while Brigette and Koch make antifade
| + | |
| - | *we need a new batch of brb80T, so we are mixing from stock at a 100:1 ratio of brb to T (thymine i think)
| + | |
| - | ** [[User:Andy Maloney|Andy Maloney]] 00:01, 6 June 2009 (EDT): The "T" is for [http://en.wikipedia.org/wiki/Paclitaxel taxol]. Taxol is an anti-cancer drug that stabilizes microtubules.
| + | |
| - | *Since koch and brigette are still making antifade, igor and I used the old stuff from the other day
| + | |
| - | **We did not see anything, just blobs of dust
| + | |
| - | *Thinking I may have fried the tubes by stabilizing with cold brb80t, we look at a "naked" sample
| + | |
| - | **plenty of tubes, there must have been a misstep during the antifade application, or just bad antifade ate our tubes
| + | |
| - | *Igor and I try once more with the "Bad AF"
| + | |
| - | **We use 20ul of tubes (instead of 5 the first 2 times)
| + | |
| - | ***We see plenty of tubes but the AF does not work, bleaches very fast
| + | |
| - | ****Interesting note: we did see tubes break apart as they became bleached
| + | |
| - | ****There was also a "flow" from the coverglass spreading out the liquid (i think Igor put more than 5ul of soln on there, but thats no big deal)
| + | |
| - | *Brigette finished her new batch of antifade and we try it with the new tubes
| + | |
| - | **After fussing around with focusing, she found the tubes and the antifade works great!
| + | |
| - | **Note: This sample has been the hardest for me to view. All the other samples this week were really easy to get in focus (kinda odd, who knows, maybe just a fluke)
| + | |
| - | **The tubes stay in view for a very long time and are quite contrastive
| + | |
| - | ***Note: both brigette and I do not notice any movement of the tubes, normally there are some "wiggles" we see from loose ends
| + | |
| - | ***They are stuck pretty hard on the glass and coverslide (as you can get a new set in view as you focus up the plane into the slide or coverglass)
| + | |
| - | **The new AF works like a charm, the images are lasting longer than any i have seen this week
| + | |
| - | **Brigette did a rough time in the bleaching and says they last ~1min 15s
| + | |
| | | | |
| - | ===See also===
| |
| - | [[User:Steven J. Koch/Notebook/Kochlab/2009/06/05/New antifade components]]
| |
| | | | |
| | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Project name
Main project page
Previous entry Next entry
