User:Linh N Le/Notebook/2009/06/05

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==[[/LABView]]==
==[[/LABView]]==
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==MT==
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*making a new batch of MT's w/ Igor while Brigette  and Koch make antifade
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*we need a new batch of brb80T, so we are mixing from stock at a 100:1 ratio of brb to T (thymine i think)
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** [[User:Andy Maloney|Andy Maloney]] 00:01, 6 June 2009 (EDT): The "T" is for [http://en.wikipedia.org/wiki/Paclitaxel taxol]. Taxol is an anti-cancer drug that stabilizes microtubules.
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*Since koch and brigette are still making antifade, igor and I used the old stuff from the other day
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**We did not see anything, just blobs of dust
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*Thinking I may have fried the tubes by stabilizing with cold brb80t, we look at a "naked" sample
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**plenty of tubes, there must have been a misstep during the antifade application, or just bad antifade ate our tubes
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*Igor and I try once more with the "Bad AF"
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**We use 20ul of tubes (instead of 5 the first 2 times)
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***We see plenty of tubes but the AF does not work, bleaches very fast
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****Interesting note: we did see tubes break apart as they became bleached
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****There was also a "flow" from the coverglass spreading out the liquid (i think Igor put more than 5ul of soln on there, but thats no big deal)
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*Brigette finished her new batch of antifade and we try it with the new tubes
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**After fussing around with focusing, she found the tubes and the antifade works great!
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**Note: This sample has been the hardest for me to view. All the other samples this week were really easy to get in focus (kinda odd, who knows, maybe just a fluke)
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**The tubes stay in view for a very long time and are quite contrastive
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***Note: both brigette and I do not notice any movement of the tubes, normally there are some "wiggles" we see from loose ends
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***They are stuck pretty hard on the glass and coverslide (as you can get a new set in view as you focus up the plane into the slide or coverglass)
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**The new AF works like a charm, the images are lasting longer than any i have seen this week
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**Brigette did a rough time in the bleaching and says they last ~1min 15s
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===See also===
 
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[[User:Steven J. Koch/Notebook/Kochlab/2009/06/05/New antifade components]]
 
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Revision as of 13:05, 29 September 2009

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