User:Linh N Le/Notebook/2009/06/05: Difference between revisions

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*Brigette finished her new batch of antifade and we try it with the new tubes
*Brigette finished her new batch of antifade and we try it with the new tubes
**After fussing around with focusing, she found the tubes and the antifade works great!  
**After fussing around with focusing, she found the tubes and the antifade works great!  
**Note: This sample has been the hardest for me to view. All the other samples this week were really easy to get in focus (kinda odd, who knows, maybe just a fluke)
**The tubes stay in view for a very long time and are quite contrastive  
**The tubes stay in view for a very long time and are quite contrastive  
***Note: both brigette and I do not notice any movement of the tubes, normally there are some "wiggles" we see from loose ends  
***Note: both brigette and I do not notice any movement of the tubes, normally there are some "wiggles" we see from loose ends  

Revision as of 14:41, 5 June 2009

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LABView

  • Larry has asked me to make a LABView VI that takes photos out of a file and loads them all to LABView
    • It also must display said photos
  • After many, many attempts, I currently have a version that does what I think he wants (feedback to follow)
  1. it will open a directory and list everything that is in it
  2. it will then load photos into LABView (currently set to look for .tif and .png)
  3. the photos are in an array
  4. if the file is not a photo, a blank will be created in the array, and then deleted afterwards
  5. i can pick out a piece of the array and look at it
  • The problems that it has are:
  1. case sensitivity: .Tif files and .tif files or .png .Png .PNG must all be coded for separately, more annoying than troublesome.
  2. display: currently only displays one photo at a time, and you must rerun the entire program to see a new photo
    • possible fix by turning the photos into a pixel array
  • The VI uses NI Vision Assistant to deal with the photos themselves

This is a screenshot of the VI's backpanel

MT

  • making a new batch of MT's w/ Igor while Brigette and Koch make antifade
  • we need a new batch of brb80T, so we are mixing from stock at a 100:1 ratio of brb to T (thymine i think)
  • Since koch and brigette are still making antifade, igor and I used the old stuff from the other day
    • We did not see anything, just blobs of dust
  • Thinking I may have fried the tubes by stabilizing with cold brb80t, we look at a "naked" sample
    • plenty of tubes, there must have been a misstep during the antifade application, or just bad antifade ate our tubes
  • Igor and I try once more with the "Bad AF"
    • We use 20ul of tubes (instead of 5 the first 2 times)
      • We see plenty of tubes but the AF does not work, bleaches very fast
        • Interesting note: we did see tubes break apart as they became bleached
        • There was also a "flow" from the coverglass spreading out the liquid (i think Igor put more than 5ul of soln on there, but thats no big deal)
  • Brigette finished her new batch of antifade and we try it with the new tubes
    • After fussing around with focusing, she found the tubes and the antifade works great!
    • Note: This sample has been the hardest for me to view. All the other samples this week were really easy to get in focus (kinda odd, who knows, maybe just a fluke)
    • The tubes stay in view for a very long time and are quite contrastive
      • Note: both brigette and I do not notice any movement of the tubes, normally there are some "wiggles" we see from loose ends
      • They are stuck pretty hard on the glass and coverslide (as you can get a new set in view as you focus up the plane into the slide or coverglass)
    • The new AF works like a charm, the images are lasting longer than any i have seen this week
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