User:Linh N Le/Notebook/2009/06/01: Difference between revisions

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*First we took an aliquot (2ul) of FITC tubulin and warmed it in the PCR
*First we took an aliquot (2ul) of FITC tubulin and warmed it in the PCR
**This caused the tubulin to polymerize into microtubules
**This caused the tubulin to polymerize into microtubules
**We also added a buffer, or enzyme, to stabilize them
**We also added a buffer, 198 ul of room temperature BRB80T, to stabilize them
*We then imaged them in the microscope
*We then imaged them in the microscope
**We saw 100's of tubes (although they are smaller than what Koch made last week http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/05/26/FITC_Microtubules_with_antifade)
**We saw 100's of tubes (although they are smaller than what Koch made last week http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/05/26/FITC_Microtubules_with_antifade)

Revision as of 15:00, 2 June 2009

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First Enty

  • Koch had mentioned that I become more "science 2.0" and so I brought it upon myself to start a notebook here on the public wiki

Microtubules

  • We made MTs today
  • First we took an aliquot (2ul) of FITC tubulin and warmed it in the PCR
    • This caused the tubulin to polymerize into microtubules
    • We also added a buffer, 198 ul of room temperature BRB80T, to stabilize them
  • We then imaged them in the microscope
  • We then made a batch with Antifade
    • The old antifade from last week was no good, but a fresh batch came out great!
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