User:Korey Griffin

Contact

Santa Cruz West cliff

Half Dome

• Korey Griffin MS
• Santa Cruz Biotechnology, Inc.
• Email me through OpenWetWare

Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.

Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success.

Methods & Techniques

Santa Cruz Mountains

Kings Canyon

Sand Point

• Antibody Basics
• Antibody Peptide Neutralization
• Antibody Protein Coupling (F:P) Ratios
• Antibody Elution Buffers
• Antibody Related Solutions & Recipes
• Antigen Retrieval Technique
• Chromatin Immunoprecipitation
• Conditioned Medium Preparation (Growth Factor/Cytokine)
• Dual Immunofluorescence Cell Staining
• Flow Cytometry prep & labeling
• Immunoprecipitation Guide
• Immunohistochemistry Paraffin
• Immunofluorescence Cell Staining
• In Vitro Kinase Assay
• Isolate Leukocytes from whole blood
• Lentivirus Technology
• Membrane Stripping and Reprobing
• Nested RT-PCR
• Nuclear Extract Preparation
• Puromycin Selection for shRNA
• shRNA Transfection
• siRNA Transfection
• Sodium Orthovanadate Activation
• Western Blotting Guide
• Western Blot Optimization
• Zinc Fixation

Links

• ATCC Cell Lines
• Organelle and Lineage Markers
• DOT guidelines
• Fluorochrome Table
• Human Protein Atlas
• Oligo Tm

North County Santa Cruz

Liberty Cap & Nevada Falls