Objective
- Read plates
- took florescence of the differ concentrations used with hydrogel pieces and determined the concentrations/mols of dye attached
Description
1µL of Dye
number of mols of dye
- starting solution: 8.9E-9 mols
- after soaking for 24hours with gel: 1.34E-9 mols
- leached in water solution after 24 hours: 4.3919E-9 mols
- 8.9E-9mols- 1.34E-9mols=7.56E-9mols of dye soaked up by gel in 24 hours
- 7.56E-9mols - 4.3919E-9mols= 3.17E-9 mols of dye in hydrogel actual
- mols butyne-1-ol sites in small piece of hydrogel (assuming 1:1 reaction): 0.000017
- Percent of dye reacted with butyne-1-ol=0.0186%
10µL of Dye
number of mols of dye
- starting solution: 8.9E-8
- after soaking for 24hours with gel: 8.8E-9 mols
- leached in water solution after 24 hours: 3.86E-9
- 8.9E-8 mols- 8.8E-9mols= 8.03E-8 mols of dye soaked up by gel in 24 hours
- 8.03E-8mols - 3.86E-9mols= 7.64E-8 mols of dye in hydrogel actual
- mols of butyne-1-ol sites in small piece of hydrogel: 0.000017
- Percent of dye reacted with butyne-1-ol= 0.45%
100µL of Dye
number of mols of dye
- starting solution: 8.9E-7mols
- after soaking for 24hours with gel: 1.608E-7mols
- leached in water solution after 24 hours: 3.856E-9 mols
- 8.9E-7 mols- 1.608E-7mols= 7.3E-7 mols of dye soaked up by gel in 24 hours
- 7.3E-7mols - 3.86E-7mols= 7.26E-7 mols of dye in hydrogel actual
- molsf butyne-1-ol in small piece of hydrogel: 0.000017
- Percent of dye reacted with butyne-1-ol= 4.27%
200µl of dye
number of mols of dye
- starting solution: 1.8E-6mols
- after soaking for 24hours with gel: 4.6E-7mols
- leached in water solution after 24 hours: 1.6E-7 mols
- 1.8E-6 mols- 4.6E-7mols= 1.34E-6 mols of dye soaked up by gel in 24 hours
- 1.34E-6mols - 1.6E-7mols= 1.18E-6 mols of dye in hydrogel actual
- number of butyne-1-ol sites in small piece of hydrogel: 0.000017
- Percent of dye reacted with butyne-1-ol= 6.94%
500µl of dye
number of mols of dye
- starting solution: 4.4E-6mols
- after soaking for 24hours with gel: 8.8E-7 mols
- leached in water solution after 24 hours: 8.4E-7 mols
- 4.4E-6 mols- 8.8E-7mols= 3.5E-6 mols of dye soaked up by gel in 24 hours
- 3.5E-6mols - 8.4E-7mols= 2.67E-6 mols of dye in hydrogel actual
- number of butyne-1-ol sites in small piece of hydrogel: 0.000017
- Percent of dye reacted with butyne-1-ol= 15.71%
Data
- Add data and results here...
Notes
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