User:Klare Lazor/Notebook/Chem-496-001/2013/04/12

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(Description)
(Description)
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**8.9E-7 mols- 1.608E-7mols=  7.3E-7 mols of dye soaked up by gel in 24 hours
**8.9E-7 mols- 1.608E-7mols=  7.3E-7 mols of dye soaked up by gel in 24 hours
** 7.3E-7mols - 3.86E-7mols= '''7.26E-7 mols of dye in hydrogel actual'''
** 7.3E-7mols - 3.86E-7mols= '''7.26E-7 mols of dye in hydrogel actual'''
-
**molsf butyne-1-ol in small piece of hydrogel: 0.000017  
+
**mols of butyne-1-ol sites in small piece of hydrogel: 0.000017  
*Percent of dye reacted with butyne-1-ol= 4.27%
*Percent of dye reacted with butyne-1-ol= 4.27%
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**1.8E-6 mols- 4.6E-7mols=  1.34E-6 mols of dye soaked up by gel in 24 hours
**1.8E-6 mols- 4.6E-7mols=  1.34E-6 mols of dye soaked up by gel in 24 hours
** 1.34E-6mols - 1.6E-7mols= '''1.18E-6 mols of dye in hydrogel actual'''
** 1.34E-6mols - 1.6E-7mols= '''1.18E-6 mols of dye in hydrogel actual'''
-
** number of butyne-1-ol sites in small piece of hydrogel: 0.000017  
+
**mols of butyne-1-ol sites in small piece of hydrogel: 0.000017  
*Percent of dye reacted with butyne-1-ol= 6.94%  
*Percent of dye reacted with butyne-1-ol= 6.94%  
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**4.4E-6 mols- 8.8E-7mols=  3.5E-6 mols of dye soaked up by gel in 24 hours
**4.4E-6 mols- 8.8E-7mols=  3.5E-6 mols of dye soaked up by gel in 24 hours
** 3.5E-6mols - 8.4E-7mols= '''2.67E-6 mols of dye in hydrogel actual'''
** 3.5E-6mols - 8.4E-7mols= '''2.67E-6 mols of dye in hydrogel actual'''
-
** number of butyne-1-ol sites in small piece of hydrogel: 0.000017  
+
** mols of butyne-1-ol sites in small piece of hydrogel: 0.000017  
*Percent of dye reacted with butyne-1-ol= 15.71%
*Percent of dye reacted with butyne-1-ol= 15.71%

Revision as of 20:14, 15 April 2013

Biomaterials Design Lab Main project page
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Objective

  • Read plates
  • took florescence of the differ concentrations used with hydrogel pieces and determined the concentrations/mols of dye attached

Description

1µL of Dye number of mols of dye

  • starting solution: 8.9E-9 mols
  • after soaking for 24hours with gel: 1.34E-9 mols
  • leached in water solution after 24 hours: 4.3919E-9 mols
    • 8.9E-9mols- 1.34E-9mols=7.56E-9mols of dye soaked up by gel in 24 hours
    • 7.56E-9mols - 4.3919E-9mols= 3.17E-9 mols of dye in hydrogel actual
    • mols butyne-1-ol sites in small piece of hydrogel (assuming 1:1 reaction): 0.000017
  • Percent of dye reacted with butyne-1-ol=0.0186%


10µL of Dye number of mols of dye

  • starting solution: 8.9E-8
  • after soaking for 24hours with gel: 8.8E-9 mols
  • leached in water solution after 24 hours: 3.86E-9
    • 8.9E-8 mols- 8.8E-9mols= 8.03E-8 mols of dye soaked up by gel in 24 hours
    • 8.03E-8mols - 3.86E-9mols= 7.64E-8 mols of dye in hydrogel actual
    • mols of butyne-1-ol sites in small piece of hydrogel: 0.000017
  • Percent of dye reacted with butyne-1-ol= 0.45%


100µL of Dye number of mols of dye

  • starting solution: 8.9E-7mols
  • after soaking for 24hours with gel: 1.608E-7mols
  • leached in water solution after 24 hours: 3.856E-9 mols
    • 8.9E-7 mols- 1.608E-7mols= 7.3E-7 mols of dye soaked up by gel in 24 hours
    • 7.3E-7mols - 3.86E-7mols= 7.26E-7 mols of dye in hydrogel actual
    • mols of butyne-1-ol sites in small piece of hydrogel: 0.000017
  • Percent of dye reacted with butyne-1-ol= 4.27%


200µl of dye number of mols of dye

  • starting solution: 1.8E-6mols
  • after soaking for 24hours with gel: 4.6E-7mols
  • leached in water solution after 24 hours: 1.6E-7 mols
    • 1.8E-6 mols- 4.6E-7mols= 1.34E-6 mols of dye soaked up by gel in 24 hours
    • 1.34E-6mols - 1.6E-7mols= 1.18E-6 mols of dye in hydrogel actual
    • mols of butyne-1-ol sites in small piece of hydrogel: 0.000017
  • Percent of dye reacted with butyne-1-ol= 6.94%


500µl of dye number of mols of dye

  • starting solution: 4.4E-6mols
  • after soaking for 24hours with gel: 8.8E-7 mols
  • leached in water solution after 24 hours: 8.4E-7 mols
    • 4.4E-6 mols- 8.8E-7mols= 3.5E-6 mols of dye soaked up by gel in 24 hours
    • 3.5E-6mols - 8.4E-7mols= 2.67E-6 mols of dye in hydrogel actual
    • mols of butyne-1-ol sites in small piece of hydrogel: 0.000017
  • Percent of dye reacted with butyne-1-ol= 15.71%

Data

  • Add data and results here...

Notes

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