User:Klare Lazor/Notebook/Chem-496-001/2013/02/25

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Current revision (00:30, 28 February 2013) (view source)
(Description)
 
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'''Procedure'''
'''Procedure'''
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To eventually attach a protein, we can not crosslink in acidic or heated conditions. Therefore, for this procedure. We will be using hydrogels instead. 4 hydrogels were prepared.  2 were made using PVOH MW = 22,000, while the other two were made using PVOH MW = 146,000 - 186,000.  For each molecular weight, one hydrogel was made with isopropanol alone, and one was made with isopropanol + glutaraldehyde.  The amounts of isopropanol used is the same as in previous trials.
To eventually attach a protein, we can not crosslink in acidic or heated conditions. Therefore, for this procedure. We will be using hydrogels instead. 4 hydrogels were prepared.  2 were made using PVOH MW = 22,000, while the other two were made using PVOH MW = 146,000 - 186,000.  For each molecular weight, one hydrogel was made with isopropanol alone, and one was made with isopropanol + glutaraldehyde.  The amounts of isopropanol used is the same as in previous trials.
# Measure out 2 samples of 1 g PVOH (MW = 22,000) and 2 samples of 1 g PVOH (MW = 146,000 - 186,000)
# Measure out 2 samples of 1 g PVOH (MW = 22,000) and 2 samples of 1 g PVOH (MW = 146,000 - 186,000)
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# 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
# 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
# 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol and 1mL glutaraldehyde (in hood) once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
# 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol and 1mL glutaraldehyde (in hood) once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
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crosslinking couldn't occur bc we didn't have time. To be done next time.
 
==Data==
==Data==

Current revision

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Objective

Hydrogels for eventual attachment of protein

Description

Procedure

To eventually attach a protein, we can not crosslink in acidic or heated conditions. Therefore, for this procedure. We will be using hydrogels instead. 4 hydrogels were prepared. 2 were made using PVOH MW = 22,000, while the other two were made using PVOH MW = 146,000 - 186,000. For each molecular weight, one hydrogel was made with isopropanol alone, and one was made with isopropanol + glutaraldehyde. The amounts of isopropanol used is the same as in previous trials.

  1. Measure out 2 samples of 1 g PVOH (MW = 22,000) and 2 samples of 1 g PVOH (MW = 146,000 - 186,000)
  2. Dissolve in 20 mL water (Dissolve on heat)
  3. Once dissolved, 0.0174 mL isopropanol was added to each sample. The samples were allowed to stir for a few minutes.
  4. 1 mL glutaraldehyde was added to the 2 hydrogels that are isopropanol + glut
  5. The hydrogels were placed in the freezer for 24 hours out for 4 hours in for 24hours and then out once again. repeated cycles.


Calculations

2 hydrogels of 22k MW PVOH and 2 hydrogels of 146-186k MW PVOH. All will have 1% isopropanol: PVOH OH groups. 1 of each will have glutaraldehyde (1mL). From previous calculations, 1g PVOH contains 1.373E22 OH groups. We want 1% of these to react w isopropanol at 1:1. (1.373E22)(0.01)=1.373E20 molecules of isopropanol. (1.373E20)/(6.022E23 molecules per mole)=2.27997E-4 moles isopropanol. (2.27994E-4 moles)(60.1 grams per mole)=0.0137 grams isopropanol. (0.0137g)/(0.786 grams per mL)=0.0174mL Isopropanol.

  1. 1g 22000mw PVOH dissolved in 20mL distilled water + 0.0174mL Isopropanol once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
  2. 1g 22000mw PVOH dissolved in 20mL distilled water + 0.0174mL Isopropanol and 1mL glutaraldehyde (in hood) once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
  3. 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
  4. 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol and 1mL glutaraldehyde (in hood) once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.

Data

  • Add data and results here...

Notes

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