User:Klare Lazor/Notebook/Chem-496-001/2012/11/19

Objective

To determine the swelling of the PVOH/glutaraldehyde films, prepare another PVOH/glutaraldehyde/porphyrin/HCl film, conduct DSC, conduct FTIR,

Description

Crosslinking Glutaraldehyde Films

1. Allow the PVOH film containing glutaraldehyde to dry completely.
2. Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant)
3. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
4. Heat solution to 70°C (temperature on hotplate should be about 160)
5. Place film in solution for one hour.
6. Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker.
7. Heat HCl solution to 70°C
8. Remove film from glutaraldehyde fixant and rinse with distilled water.
9. Place film into HCl solution for 30 minutes.
10. Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL)
11. After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes.
12. Use tweezers to remove the film from the sodium bicarbonate solution.
13. Rinse film with deionized water and dry film on paper towel.

Swelling

1. One of the PVOH/glutaraldehyde films that had been crosslinked on 11/16 was removed from water.
2. The mass of the film was measured.
3. The film was dried in an oven set to 90°C for about 30 minutes and then was left to air dry for 1 week.

Porphyrin Film Preparation

• 3 porphyrin films were prepared. Two of the films contained the HCl seen in step 2. The HCl portion of step 2 was omitted.

1. 1 g of PVOH was dissolved in 7 mL H₂O on a heated hot plate.
2. Once the PVOH dissolved, 1 mL of glutaraldehyde, 1 mL of porphyrin, and 2-3 drops of HCl was added.
3. The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate for 1 week.

Data

• Add data and results here...

Notes

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