User:Klare Lazor/Notebook/Chem-496-001/2012/11/16: Difference between revisions
From OpenWetWare
Klare Lazor (talk | contribs) (Autocreate 2012/11/16 Entry for User:Klare_Lazor/Notebook/Chem-496-001) |
Klare Lazor (talk | contribs) |
||
Line 12: | Line 12: | ||
==Description== | ==Description== | ||
Crosslinking Glutaraldehyde Films | |||
#Allow the PVOH film containing glutaraldehyde to dry completely. | |||
#Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) | |||
#Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | |||
#Heat solution to 70°C (temperature on hotplate should be about 160) | |||
#Place film in solution for one hour. | |||
#Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker. | |||
#Heat HCl solution to 70°C | |||
#Remove film from glutaraldehyde fixant and rinse with distilled water. | |||
#Place film into HCl solution for 30 minutes. | |||
#Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL) | |||
#After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes. | |||
#Use tweezers to remove the film from the sodium bicarbonate solution. | |||
#Rinse film with deionized water and dry film on paper towel. | |||
==Data== | ==Data== |
Revision as of 11:09, 19 November 2012
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveDescriptionCrosslinking Glutaraldehyde Films
Data
NotesThis area is for any observations or conclusions that you would like to note.
|