User:Klare Lazor/Notebook/Chem-496-001/2012/10/26: Difference between revisions
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# The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday. | # The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday. | ||
*Save each solution in a glass container for testing | *Save each solution in a glass container for testing | ||
* each solution was 100ml water for sodium bicarbonate, 100ml of fixant, and 99ml water and 1 ml hcl | |||
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# Once the PVOH dissolved, 1 mL of glutaraldehyde was added. | # Once the PVOH dissolved, 1 mL of glutaraldehyde was added. | ||
# The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday. | # The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday. | ||
<u>Crosslinking Glutaraldehyde Films</u> | |||
# Allow the PVOH film containing glutaraldehyde to dry completely. | |||
# Prepare a solution of 100 mL H<sub>2</sub>O, 2 mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20 g Na<sub>2</sub>SO<sub>4</sub> in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) | |||
# Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | |||
# Heat solution to 70°C (temperature on hotplate should be about 160) | |||
# Place film in solution for one hour. | |||
# Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker. | |||
# Heat HCl solution to 70°C | |||
# Remove film from glutaraldehyde fixant and rinse with distilled water. | |||
# Place film into HCl solution for 30 minutes. | |||
# Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL) | |||
# After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes. | |||
# Use tweezers to remove the film from the sodium bicarbonate solution. | |||
# Rinse film with deionized water and place in a beaker with distilled water until it is used. | |||
Revision as of 12:20, 26 October 2012
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ObjectiveDescriptionPorphyrin Film Preparation
UV-Vis of porhyrin ring concentration curve Data
NotesThis area is for any observations or conclusions that you would like to note.
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