User:Klare Lazor/Notebook/Chem-496-001/2012/10/15

Objective

Description

remake control: 1mL control glutaraldehyde and 4 other films remake and 1ml glultaraldehye, as well as 1ml of porphyrin exact amount of water so know concentration of solution.

Na+ electrode: 1) wait hit measure 2) wait till stabilizes 3) higher concentration the more negative, ionic strength adjuster 4)Please refer to the ISE protocol on 2012/09/28 for how ISE measurements were taken.

• 3 mL of the solutions were added to 0.1 mL of ionic strength adjuster (4 M solution containing NaOH) to adjust the pH to a value between 8 and 10.
  • Sodium ionic strength adjuster provided by thermo scientific 4M
• The solutions were allowed to equilibrate with the electrode for 2 min.

Data

Making Glutaraldehyde Films

4 films were made:

1. PVOH/glutaraldehyde
2. PVOH/Na-MT/glutaraldehyde
3. PVOH/100% CEC Clay/glutaraldehyde
4. PVOH/porphyrin/glutaraldehyde

• Film 1 was made by dissolving 1 g PVOH in 7 mL of water. Once all the PVOH had dissolved, 1 mL of glutaraldehyde was added. The solution was stirred for 5 minutes and then was poured into a dish and allowed to evaporate until Wednesday.

Actual amount of PVOH = 1.0059 g

• Film 2 was made by dissolving 1 g PVOH and 0.1 g Na-MT in 7 mL of water. Once they had dissolved, 1 mL of glutaraldehyde was added. The solution was allowed to mix for 5 minuntes and then was poured into a dish and allowed to evaporate until Wednesday.

Actual amount of PVOH = 1.0021 g, actual amount of Na-MT = 0.1016 g

• Film 3 was made by dissolving 1 g PVOH and 0.1 g 100% CEC clay in 7 mL of water. Once they had dissolved, 1 mL of glutaraldehyde was added. The solution was allowed to stir for 5 minuntes and then was poured into a dish and allowed to evaporate until Wednesday.

Actual amount of PVOH added = 1.0014 g, actual amount of 100% CEC clay = 0.1004 g.

• Film 4 was made by dissolving 1 g PVOH in 7 mL of water. Once it dissolved, 1 mL of glutaraldehyde and 1 mL of porphyrin was added. The solution was allowed to mix for 5 minutes and then was poured into a dish and allowed to evaporate until Wednesday.

Films

1. PVOH and glutaraldehyde 7ml of h20
   1. PVOH~1.0059g
2. PVOH and glutaraldehye and porphrin 7ml of h20
   1. PVOH~1.0059g
3. PVOH and NA-MT and glutaraldheyhe 7ml of h20
   1. PVOH~1.0021g
   2. PVOH~.1016g
4. PVOH and 100% surfactant and glutaraldehyde 7 ml of h20
   1. PVOH and 1.0014g
   2. 100% surfactant clay .1004g

Making Glutaraldehyde Films

• 4 films were made - (1)PVOH/glutaraldehyde, (2)PVOH/Na-MT/glutaraldehyde, (3)PVOH/100% CEC Clay/glutaraldehyde, and (4)PVOH/porphyrin/glutaraldehyde

1. Film 1 was made by dissolving 1 g PVOH in 7 mL of water. Once all the PVOH had dissolved, 1 mL of glutaraldehyde was added. The solution was stirred for 5 minutes and then was poured into a dish and allowed to evaporate until Wednesday.
   • Actual amount of PVOH = 1.0059 g
2. Film 2 was made by dissolving 1 g PVOH and 0.1 g Na-MT in 7 mL of water. Once they had dissolved, 1 mL of glutaraldehyde was added. The solution was allowed to mix for 5 minuntes and then was poured into a dish and allowed to evaporate until Wednesday.
   • Actual amount of PVOH = 1.0021 g, actual amount of Na-MT = 0.1016 g
3. Film 3 was made by dissolving 1 g PVOH and 0.1 g 100% CEC clay in 7 mL of water. Once they had dissolved, 1 mL of glutaraldehyde was added. The solution was allowed to stir for 5 minuntes and then was poured into a dish and allowed to evaporate until Wednesday.
   • Actual amount of PVOH added = 1.0014 g, actual amount of 100% CEC clay = 0.1004 g.
4. Film 4 was made by dissolving 1 g PVOH in 7 mL of water. Once it dissolved, 1 mL of glutaraldehyde and 1 mL of porphyrin was added. The solution was allowed to mix for 5 minutes and then was poured into a dish and allowed to evaporate until Wednesday.

Determining the Sodium Ion Content

• The sodium ion content of the filtrates collected on

1. An 0.1 mL of ionic strength adjuster (4M solution containing NaOH) was added to a 3 mL of each solution to be tested, so that the pH of each solution would fall in the range of pH = 8 to 10.
2. The electrode was placed into each of the solutions and were allowed to equilibrate for 2 minutes.
3. This procedure was done for all of standards

Notes

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