User:Klare Lazor/Notebook/Chem-496-001/2012/10/15: Difference between revisions
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==Description== | ==Description== | ||
remake control: 1mL control glutaraldehyde and 4 other films remake and 1ml glultaraldehye, as well as 1ml of porphyrin exact amount of water so know concentration of solution. | |||
Na+ electrode: | |||
1) wait hit measure | |||
2) wait till stabilizes | |||
3) higher concentration the more negative, ionic strength adjuster | |||
4)Please refer to the ISE protocol on 2012/09/28 for how ISE measurements were taken. | |||
* 3 mL of the solutions were added to 0.1 mL of ionic strength adjuster (4 M solution containing NaOH) to adjust the pH to a value between 8 and 10. | |||
**Sodium ionic strength adjuster provided by thermo scientific 4M | |||
* The solutions were allowed to equilibrate with the electrode for 2 min. | |||
==Data== | ==Data== | ||
* | |||
'''Making Glutaraldehyde Films''' | |||
4 films were made: | |||
#PVOH/glutaraldehyde | |||
#PVOH/Na-MT/glutaraldehyde | |||
#PVOH/100% CEC Clay/glutaraldehyde | |||
#PVOH/porphyrin/glutaraldehyde | |||
*Film 1 was made by dissolving 1 g PVOH in 7 mL of water. Once all the PVOH had dissolved, 1 mL of glutaraldehyde was added. The solution was stirred for 5 minutes and then was poured into a dish and allowed to evaporate until Wednesday. | |||
Actual amount of PVOH = 1.0059 g | |||
*Film 2 was made by dissolving 1 g PVOH and 0.1 g Na-MT in 7 mL of water. Once they had dissolved, 1 mL of glutaraldehyde was added. The solution was allowed to mix for 5 minuntes and then was poured into a dish and allowed to evaporate until Wednesday. | |||
Actual amount of PVOH = 1.0021 g, actual amount of Na-MT = 0.1016 g | |||
*Film 3 was made by dissolving 1 g PVOH and 0.1 g 100% CEC clay in 7 mL of water. Once they had dissolved, 1 mL of glutaraldehyde was added. The solution was allowed to stir for 5 minuntes and then was poured into a dish and allowed to evaporate until Wednesday. | |||
Actual amount of PVOH added = 1.0014 g, actual amount of 100% CEC clay = 0.1004 g. | |||
*Film 4 was made by dissolving 1 g PVOH in 7 mL of water. Once it dissolved, 1 mL of glutaraldehyde and 1 mL of porphyrin was added. The solution was allowed to mix for 5 minutes and then was poured into a dish and allowed to evaporate until Wednesday. | |||
'''Films''' | |||
#PVOH and glutaraldehyde 7ml of h20 | |||
##PVOH~1.0059g | |||
#PVOH and glutaraldehye and porphrin 7ml of h20 | |||
##PVOH~1.0059g | |||
#PVOH and NA-MT and glutaraldheyhe 7ml of h20 | |||
##PVOH~1.0021g | |||
##PVOH~.1016g | |||
#PVOH and 100% surfactant and glutaraldehyde 7 ml of h20 | |||
##PVOH and 1.0014g | |||
##100% surfactant clay .1004g | |||
<u>Making Glutaraldehyde Films</u> | |||
* 4 films were made - (1)PVOH/glutaraldehyde, (2)PVOH/Na-MT/glutaraldehyde, (3)PVOH/100% CEC Clay/glutaraldehyde, and (4)PVOH/porphyrin/glutaraldehyde | |||
# Film 1 was made by dissolving 1 g PVOH in 7 mL of water. Once all the PVOH had dissolved, 1 mL of glutaraldehyde was added. The solution was stirred for 5 minutes and then was poured into a dish and allowed to evaporate until Wednesday. | |||
#* Actual amount of PVOH = 1.0059 g | |||
# Film 2 was made by dissolving 1 g PVOH and 0.1 g Na-MT in 7 mL of water. Once they had dissolved, 1 mL of glutaraldehyde was added. The solution was allowed to mix for 5 minuntes and then was poured into a dish and allowed to evaporate until Wednesday. | |||
#* Actual amount of PVOH = 1.0021 g, actual amount of Na-MT = 0.1016 g | |||
#Film 3 was made by dissolving 1 g PVOH and 0.1 g 100% CEC clay in 7 mL of water. Once they had dissolved, 1 mL of glutaraldehyde was added. The solution was allowed to stir for 5 minuntes and then was poured into a dish and allowed to evaporate until Wednesday. | |||
#* Actual amount of PVOH added = 1.0014 g, actual amount of 100% CEC clay = 0.1004 g. | |||
# Film 4 was made by dissolving 1 g PVOH in 7 mL of water. Once it dissolved, 1 mL of glutaraldehyde and 1 mL of porphyrin was added. The solution was allowed to mix for 5 minutes and then was poured into a dish and allowed to evaporate until Wednesday. | |||
<u>Determining the Sodium Ion Content</u> | |||
* The sodium ion content of the filtrates collected on | |||
# An 0.1 mL of ionic strength adjuster (4M solution containing NaOH) was added to a 3 mL of each solution to be tested, so that the pH of each solution would fall in the range of pH = 8 to 10. | |||
# The electrode was placed into each of the solutions and were allowed to equilibrate for 2 minutes. | |||
# This procedure was done for all of standards | |||
==Notes== | ==Notes== |
Revision as of 09:14, 26 October 2012
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ObjectiveDescriptionremake control: 1mL control glutaraldehyde and 4 other films remake and 1ml glultaraldehye, as well as 1ml of porphyrin exact amount of water so know concentration of solution.
DataMaking Glutaraldehyde Films 4 films were made:
Actual amount of PVOH = 1.0059 g
Actual amount of PVOH = 1.0021 g, actual amount of Na-MT = 0.1016 g
Actual amount of PVOH added = 1.0014 g, actual amount of 100% CEC clay = 0.1004 g.
NotesThis area is for any observations or conclusions that you would like to note.
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