User:Klare Lazor/Notebook/Chem-496-001/2012/02/22: Difference between revisions

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==Objective==
==Objective==
*tested bsa+ gold and dye with fluorescence (400 nm and 280)
*tested bsa+ gold and dye with fluorescence 1st batch (400 nm and 280)
*made more bsa + dye at same protocol from the feb 14
*made more bsa + dye at same protocol from the feb 14 second batch
* made a sample of bsa+ buffer for the concentration curve as a standard  
* made a sample of bsa+ buffer for the concentration curve as a standard  
** 406µl buffer/ 2776 µL = x / 10000µL
** 406µl buffer/ 2776 µL = x / 10000µL

Revision as of 11:45, 28 February 2012

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Objective

  • tested bsa+ gold and dye with fluorescence 1st batch (400 nm and 280)
  • made more bsa + dye at same protocol from the feb 14 second batch
  • made a sample of bsa+ buffer for the concentration curve as a standard
    • 406µl buffer/ 2776 µL = x / 10000µL
    • (15µM)(2.7)=(x)(3.18) new concentration in the BSA+Dye+buffer solution = 12.7 µM of BSA in buffer +dye

Description

  • diluted gold to 30 µL gold+BSA+dye+buffer +1,000 µL buffer
  • diluted gold to 200 µL gold+BSA+dye+buffer + 1,000 µL buffer
  • BSA+buffer - diluted 30 µL BSA+buffer + 1,000 µL buffer

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


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