User:Khyra A. Neal/Notebook/Experimental Biological Chemistry II Notebook/2015/03/03: Difference between revisions

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==Entry title==
==March 3, 2015==
* Insert content here...
 
==Objective==
# Stock Solution Preparation
# Prepare Citrate Gold Nanoparticles
# Determine Concentration of Citrate AuNPs
# Start new reaction with citrate nanoparticles
 
 
==Description==
# Stock Solution Preparation
## 1% wt HAuCl<sub>4</sub>
##* Dissolve 0.1201 g HAuCl<sub>4</sub> in 10 mL H<sub>2</sub>O
## 38.8 mM Sodium Citrate
##* Dissolve 0.11601 g sodium citrate in 10 mL H<sub>2</sub>O
## 0.075% wt. NaBH<sub>4</sub>
##* Dissolve 0.00357 g NaBH<sub>4</sub> in 5 mL 38.8 mM Sodium Citrate solution that was prepared previously
# Citrate Nanoparticles
## 90 mL H<sub>2</sub>O to 100 mL volumetric flask
## Add 1 mL 1% wt HAuCl<sub>4</sub> and stir for 1 minute
## Add  2 mL 38.8 mM Sodium Citrate and stir for 1 minute
## Add 1 mL 0.075% wt. NaBH<sub>4</sub> and stir for 5 minutes
##* The solution turned a deep burgundy and was stored in a screw top bottle for future use.
# The concentration of citrate AuNPs were determined using a method described by [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf Wolfgang Haiss] in 'Determination of Size and Concentration of Gold Nanoparticles using UV/vis Spectra'
# Begin New Reaction
#* A new reaction was setup and the methods are described on [http://openwetware.org/wiki/User:Khyra_A._Neal/Notebook/Experimental_Biological_Chemistry_II_Notebook/2015/02/24 February 24,2015]
#* The method was edited by adding 2 mL citrate AuNPs instead of 30:1 Au:Lys NPs
 




Revision as of 18:18, 6 March 2015

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March 3, 2015

Objective

  1. Stock Solution Preparation
  2. Prepare Citrate Gold Nanoparticles
  3. Determine Concentration of Citrate AuNPs
  4. Start new reaction with citrate nanoparticles


Description

  1. Stock Solution Preparation
    1. 1% wt HAuCl4
      • Dissolve 0.1201 g HAuCl4 in 10 mL H2O
    2. 38.8 mM Sodium Citrate
      • Dissolve 0.11601 g sodium citrate in 10 mL H2O
    3. 0.075% wt. NaBH4
      • Dissolve 0.00357 g NaBH4 in 5 mL 38.8 mM Sodium Citrate solution that was prepared previously
  2. Citrate Nanoparticles
    1. 90 mL H2O to 100 mL volumetric flask
    2. Add 1 mL 1% wt HAuCl4 and stir for 1 minute
    3. Add 2 mL 38.8 mM Sodium Citrate and stir for 1 minute
    4. Add 1 mL 0.075% wt. NaBH4 and stir for 5 minutes
      • The solution turned a deep burgundy and was stored in a screw top bottle for future use.
  3. The concentration of citrate AuNPs were determined using a method described by Wolfgang Haiss in 'Determination of Size and Concentration of Gold Nanoparticles using UV/vis Spectra'
  4. Begin New Reaction
    • A new reaction was setup and the methods are described on February 24,2015
    • The method was edited by adding 2 mL citrate AuNPs instead of 30:1 Au:Lys NPs



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