User:Khyra A. Neal/Notebook/Experimental Biological Chemistry II Notebook/2015/01/28

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January 28, 2015

1. Gold Colloid Concentration Determination

The method to determine the size and concentration of the nanoparticles was described in 'Determination of Size and Concentration of Gold Nanoparticles from UV/Vis Spectra' by Wolfgang Haiss

  • Measure the absorbance of the 30:1 Au:Lys colloid to determine the surface plasmon resonance peak (SPR).
  • Run UV/Vis between 800 - 400 nm
  • The SPR peak was found to be 529 nm.
  • The ratio of the absorbance at the SPR peak (A529) and absorbance at 450 nm (A450) was used to determine the diameter of the nanoparticles.
  • The diameter found was used to determine the molar absorptivity at 450 nm (ε450)
  • The concentration was calculated using: c= A450 / ε450
Sample SPR Wavelength SPR Abs Abs 450 Abs Water at 450 SPR Corrected Abs Water at 529 A450 Corrected Aspr/A450 Diameter (nm) ε450 Concentration(M) Concentration (nM)
1 529 0.85 0.596 0.058 0.796 0.054 0.538 1.479553903 9.5 5.65E+07 9.52E-09 9.52
2 529 0.844 0.596 0.058 0.79 0.054 0.538 1.468401487 9.25 5.40E+07 9.96E-09 9.96
3 529 0.888 0.627 0.058 0.834 0.054 0.569 1.46572935 9.25 5.40E+07 1.05E-08 10.54
4 529 0.843 0.594 0.058 0.789 0.054 0.536 1.472014925 9.25 5.40E+07 9.93E-09 9.93
5 529 0.873 0.617 0.058 0.819 0.054 0.559 1.465116279 9.25 5.40E+07 1.04E-08 10.35
6 529 0.892 0.639 0.058 0.838 0.054 0.581 1.442340792 8.5 3.93E+07 1.48E-08 14.78
7 529 0.865 0.614 0.058 0.811 0.054 0.556 1.458633094 9 4.43E+07 1.26E-08 12.55
8 529 0.896 0.633 0.058 0.842 0.054 0.575 1.464347826 9 4.43E+07 1.30E-08 12.98
9 529 0.883 0.624 0.058 0.829 0.054 0.566 1.464664311 9 4.43E+07 1.28E-08 12.78
10 529 0.861 0.611 0.058 0.807 0.054 0.553 1.459312839 9 4.43E+07 1.25E-08 12.48

The average concentration of the AuNPs was found to be 11.59 nM. It is to be assumed that the fibers in solution will have the same concentration.


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