User:Khyra A. Neal/Notebook/Chem 571/2014/11/11: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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| align="center" style="background:#f0f0f0;"|'''Substance'''
| align="center" style="background:#f0f0f0;"|'''Substance'''
| align="center" style="background:#f0f0f0;"|'''Potential measurement (mV)'''
| align="center" style="background:#f0f0f0;"|'''Potential (mV)'''
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| 5 mM CaCl<sub>2</sub> (1) || 59.1
| 5 mM CaCl<sub>2</sub> (1) || 59.1

Latest revision as of 00:36, 27 September 2017

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November 11, 2014

Tasklist

1. Prepare new 0.12 g/L Lysozyme Curve

  • between 20 μL-100 μL Lysozyme
  • Prepare new 0.12 g/L Lysozyme
    • Add 0.006 g Lys to 50 mL H2O
  • For 1 mL Solutions add:
    • 20 μL Lysozyme,
    • 200 μL Bradford Reagent (diluted 1:3)
    • 780 μL 50 mM Tris/ 50 mM NaCl buffer
      • Amount of Lysozyme added was increased in 20 μL increments and the amount of buffer decreased in 20 μL increments until the total amount of Lysozyme added totaled 100 μL
  • Ran UV-Vis between 400 nm- 800 nm

2. Potential Measurements of 42 μM Lysozyme (0.6 g/L) dialyzed against 5-45 mM CaCl2

Ca2+ ISE

Substance Potential (mV)
5 mM CaCl2 (1) 59.1
Lysozyme 1 53.9
15 mM CaCl2 (2) 70.5
Lysozyme 2 65.6
25 mM CaCl2 (3) 72.2
Colloid 3 71.8
35 mM CaCl2 (4) 75.9
Colloid 4 78.6
45 mM CaCl2 (5) 79.1
Colloid 5 82.7



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