User:Khyra A. Neal/Notebook/Chem 571/2014/10/22: Difference between revisions
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* 700 μL 50 mM Tris/ 50 mM NaCl buffer | * 700 μL 50 mM Tris/ 50 mM NaCl buffer | ||
* Run UV-Vis between 400 nm and 800 nm | * Run UV-Vis between 400 nm and 800 nm | ||
[[Image:Bradford_of_Colloid_in_Cacl2.jpg]] | |||
3. UV-Vis and Fluorescence | 3. UV-Vis and Fluorescence |
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Ocotober 22, 2014Tasklist
Bradford Analysis of Extracted Colloid Solutions
3. UV-Vis and Fluorescence There are no peaks at 280 nm for the dialyzed colloid solutions because phenylalanine, tryptophan, and tyrosine are reducing agents to gold. There is no free protein remaining in solution after dialysis. The diagrams below confirm the above statement.
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