User:Khyra A. Neal/Notebook/Chem 571/2014/10/21

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October 21, 2014

Tasklist

1. Preparation of Dialysis for 30:1 Colloid vs CaCl2

2. Prepare ISE for Iodide

3. Determine Molecular weight of Electrophoresis gel from October 15, 2014

4. Prepare 10 mL 3 M NaCl

  • Carefully remove yellow plastic covering on Ag/AgCl electrode (as instructed)
  • Place electrode in 15 mL falcon tube with enough NaCl solution to match volume in electrode
  • cover and store upright until needed

5. Prepare 50 mL 4 M KNO3

  • Add KNO3 to outer double junction cell, filling narrow tube + ~ 0.5 inches further

Place electrode in small vial with enough KNO3 solution to cover the vycor tip

  • Prepare 50 mL 0.01 M KI + 0.1 M KNO3
  • Cut a short piece (~ 3 in) of silver wire
  • Place in KI/KNO3 solution
  • Electrolyze the solution using the power supply
  • The standard reduction potential for Ag/AgI is -0.15 V
  • Oxidation occurs at the anode, which is connected to the red lead
  • Electrolyze at low power (low current) until the wire appears coated

6. Test your electrodes with a range of KI solutions

  • The Ag/AgCl electrode slides inside of the KNO3 bridge solution (this is a double junction electrode)
  • The Ag/AgI wire can be connected directly to a multimeter using alligator clips
  • You will continue to connect your Ag wire to the red lead on the multimeter



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