User:Khyra A. Neal/Notebook/Chem 571/2014/10/21: Difference between revisions
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|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==October 21, 2014== | ||
* | ===Tasklist=== | ||
1. Preparation of Dialysis for 30:1 Colloid vs CaCl2 | |||
2. Prepare ISE for Iodide | |||
3. Determine Molecular weight of Electrophoresis gel from [http://openwetware.org/wiki/User:Khyra_A._Neal/Notebook/Chem_571/2014/10/15 October 15, 2014] | |||
By comparison with Precision Plus Protein Standards, the molecular weight of all samples was close to 15 KD, slightly lower, as expected for lysozyme compounds with MW=14,300 KD. | |||
There was a very small quantity of unknown A and colloid in the gel. The very faint mark observed in well 3 could even be considered to be bleed-over from the 30:1 colloid in well 4. Therefore, electrophoresis was inconclusive with respect to the molecular weight of unknown A. | |||
4. Prepare 10 mL 3 M NaCl | |||
* Carefully remove yellow plastic covering on Ag/AgCl electrode (as instructed) | |||
* Place electrode in 15 mL falcon tube with enough NaCl solution to match volume in electrode | |||
* cover and store upright until needed | |||
5. Prepare 50 mL 4 M KNO3 | |||
* Add KNO3 to outer double junction cell, filling narrow tube + ~ 0.5 inches further | |||
Place electrode in small vial with enough KNO3 solution to cover the vycor tip | |||
* Prepare 50 mL 0.01 M KI + 0.1 M KNO3 | |||
* Cut a short piece (~ 3 in) of silver wire | |||
* Place in KI/KNO3 solution | |||
* Electrolyze the solution using the power supply | |||
* The standard reduction potential for Ag/AgI is -0.15 V | |||
* Oxidation occurs at the anode, which is connected to the red lead | |||
* Electrolyze at low power (low current) until the wire appears coated | |||
6. Test your electrodes with a range of KI solutions '''NEED TO DO''' | |||
* The Ag/AgCl electrode slides inside of the KNO3 bridge solution (this is a double junction electrode) | |||
* The Ag/AgI wire can be connected directly to a multimeter using alligator clips | |||
* You will continue to connect your Ag wire to the red lead on the multimeter | |||
7. I<sup>-</sup> ISE Calibration Curve | |||
[[Image:KI_Calibration_Curve.jpg]] | |||
==Stock Solution Preparation== | |||
1. The previous KI solutions were discarded because there was an error with the dilutions that were prepared. All new KI concentrations were prepared | |||
* 50 mM KI = '''0.83 g KI in 100 mL H<sub>2</sub>O''' | |||
* All dilutions were prepared from 50 mM stock KI | |||
** 25 mL 25 mM KI | |||
** 25 mL 10 mM KI | |||
** 25 mL 5 mM KI | |||
** 25 mL 2 mM KI | |||
2. 10 mM NH<sub>4</sub>SCN | |||
* '''0.7612 g NH<sub>4</sub>SCN in 1 L H<sub>2</sub>O''' | |||
3. 75 mM AgNO<sub>3</sub> | |||
* '''0.637 g AgNO<sub>3</sub> in 50 mL H<sub>2</sub>O''' | |||
Latest revision as of 00:27, 27 September 2017
Project name | Main project page Previous entry Next entry |
October 21, 2014Tasklist1. Preparation of Dialysis for 30:1 Colloid vs CaCl2 2. Prepare ISE for Iodide 3. Determine Molecular weight of Electrophoresis gel from October 15, 2014 By comparison with Precision Plus Protein Standards, the molecular weight of all samples was close to 15 KD, slightly lower, as expected for lysozyme compounds with MW=14,300 KD. There was a very small quantity of unknown A and colloid in the gel. The very faint mark observed in well 3 could even be considered to be bleed-over from the 30:1 colloid in well 4. Therefore, electrophoresis was inconclusive with respect to the molecular weight of unknown A. 4. Prepare 10 mL 3 M NaCl
5. Prepare 50 mL 4 M KNO3
Place electrode in small vial with enough KNO3 solution to cover the vycor tip
6. Test your electrodes with a range of KI solutions NEED TO DO
7. I- ISE Calibration Curve Stock Solution Preparation1. The previous KI solutions were discarded because there was an error with the dilutions that were prepared. All new KI concentrations were prepared
2. 10 mM NH4SCN
3. 75 mM AgNO3
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