October 14, 2014
Tasklist
1. SDS Page #2
- Mix 10 μL 0.6 g/L lysozyme with 10μL SDS-PAGE sample buffer in 1.5 mL centrifuge vial
- Mix 10 μL 0.12 g/L lysozyme with 10μL SDS-PAGE sample buffer in 1.5 mL centrifuge vial
- Mix 10 μL 30:1 Au/lysozyme colloid with 10μL SDS-PAGE sample buffer in 1.5 mL centrifuge vial
- Mix 10 μL 0.12 g/L unknown protein with 10 μL SDS-PAGE sample buffer in 1.5 mL centrifuge vial
- Place in heating block (set at 90 °C) for 5 minutes
- Store in refrigerator overnight
2. Bradford Analysis
- Analysis of Solutions that were extracted from wells that set for one week on October 8, 2014
- Remove 20 μL of solution from each chamber (10 in all) and run Bradford analysis
- Bradford reagent should be diluted 1:3 with 50mM Tris/50mM NaCl
- Dilute to 1 mL by adding 780 μL stock 50 mM Tris/NaCl
- Measure UV-Vis from 400 nm - 800 nm using polystyrene cuvettes.
- Run blank of Tris/NaCl buffer
- Run UV-Vis of undialyzed 0.6 g/L Lysozyme solution with Bradford reagent
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