User:Khyra A. Neal/Notebook/Chem 571/2014/10/07: Difference between revisions

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==Entry title==
==October 7, 2014==
* Insert content here...


===Tasklist===
1. Solution Preparation
* 42 μM = 0.6 g/L Lysozyme
** add 0.015 g Lysozyme to 25 mL deionized water
* 30:1 lysozyme-gold colloid
** 1 mL 250 mM HAuCl<sub>4</sub> , 2 mL 42 μM (0.6 g/L) Lysozyme, and 7 mL HPLC-grade H<sub>2</sub>O
** Place in oven for 4 hours
* 50 mm NaCl 50 mM Tris buffer solution was made by adding:
** 0.3030g of Tris buffer
** 0.1461g of NaCl
**Add to 50 mL distilled water
2.  Bradford Analysis
* Solutions were prepared on [http://openwetware.org/wiki/User:Khyra_A._Neal/Notebook/Chem_571/2014/10/01 October 1, 2014]
** 20 μL sample solution
** 200 μL Bradford Reagent (diluted 1:3 with Tris buffer)
** 780 μL 50 mM Tris/ 50 mM NaCl Buffer
*** Solutions were vortexed in a 1.5 mL centrifuge tube and equilibrated for 5 minutes
*** Ran UV-Vis between 400-800 nm using PS cuvettes
*** An Undialyzed Lysozyme Bradford analysis was also ran.
*Transfer remaining dialyzed solutions to an extraction vial.
3.  pH Measurements of H<sup>+</sup> containing solutions
<u>pH measurements</u>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''pH'''
|-
| 0.25 mM HCl (4) ||  8.062
|-
| Lysozyme (4) || 8.134
|-
|}
4.  Ca<sup>2+</sup> ISE of Ca<sup>2+</sup> containing solutions
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Substance'''
| align="center" style="background:#f0f0f0;"|'''Potential (mV)'''
|-
| Colloid Sample 1 || 73.5
|-
| 50 mM CaCl<sub>2</sub> (1) || 74.9
|-
| Colloid Sample 2 || 51.2
|-
| 500 μM CaCl<sub>2</sub> || 91.4
|}
 


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Revision as of 08:01, 19 October 2014

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October 7, 2014

Tasklist

1. Solution Preparation

  • 42 μM = 0.6 g/L Lysozyme
    • add 0.015 g Lysozyme to 25 mL deionized water
  • 30:1 lysozyme-gold colloid
    • 1 mL 250 mM HAuCl4 , 2 mL 42 μM (0.6 g/L) Lysozyme, and 7 mL HPLC-grade H2O
    • Place in oven for 4 hours
  • 50 mm NaCl 50 mM Tris buffer solution was made by adding:
    • 0.3030g of Tris buffer
    • 0.1461g of NaCl
    • Add to 50 mL distilled water


2. Bradford Analysis

  • Solutions were prepared on October 1, 2014
    • 20 μL sample solution
    • 200 μL Bradford Reagent (diluted 1:3 with Tris buffer)
    • 780 μL 50 mM Tris/ 50 mM NaCl Buffer
      • Solutions were vortexed in a 1.5 mL centrifuge tube and equilibrated for 5 minutes
      • Ran UV-Vis between 400-800 nm using PS cuvettes
      • An Undialyzed Lysozyme Bradford analysis was also ran.
  • Transfer remaining dialyzed solutions to an extraction vial.

3. pH Measurements of H+ containing solutions

pH measurements

Sample pH
0.25 mM HCl (4) 8.062
Lysozyme (4) 8.134

4. Ca2+ ISE of Ca2+ containing solutions

Substance Potential (mV)
Colloid Sample 1 73.5
50 mM CaCl2 (1) 74.9
Colloid Sample 2 51.2
500 μM CaCl2 91.4



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