User:Khyra A. Neal/Notebook/Chem 571/2014/09/16: Difference between revisions
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==Dialysis== | ==Dialysis== | ||
1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube | 1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube | ||
* 3500 g/mol MWCO tubing in approximately | * 3500 g/mol MWCO tubing in approximately 150 mL of each "soak" solution | ||
** NaCl | ** NaCl | ||
** Glycine | ** Glycine | ||
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*25k g/mol MWCO tubing in Glycine soak | *25k g/mol MWCO tubing in Glycine soak | ||
2. Dialyze 4 mL of Lysozyme colloid solution in 3500 g/mol MWCO tubing for glycine soak. | 2. Dialyze 4 mL of Lysozyme colloid solution (30:1 [Au]:[Lysozyme]) in 3500 g/mol MWCO tubing for glycine soak. | ||
3. Beakers were labeled, covered with parafilm, and placed on a shaker at low speed overnight. | |||
==pH and Conductivity== | ==pH and Conductivity== | ||
Revision as of 21:47, 17 September 2014
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September 16, 2014Tasklist1. Prepare 500 mL 50mM NaCl
2. Prepare 1 L 50 mM Glycine Buffer at pH 3.5
3. Prepare 25 mL 1 g/L Lysozyme
Dialysis1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube
2. Dialyze 4 mL of Lysozyme colloid solution (30:1 [Au]:[Lysozyme]) in 3500 g/mol MWCO tubing for glycine soak. 3. Beakers were labeled, covered with parafilm, and placed on a shaker at low speed overnight. pH and Conductivity
NOTE The 0.1 mM solution was omitted because all of the data measured were outliers. The omitted data is shown in the table but is NOT included in the graphs. The outlier is indicated on the table by *.
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