User:Khyra A. Neal/Notebook/Chem 571/2014/09/16: Difference between revisions
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==<font color=#0f1028></font>== | |||
{|style="width:300px" | |||
|<u>Glycine (mM) </u> | |||
|<u>pH</u> | |||
|<u>Conductivity (μS/cm) </u> | |||
|- | |||
|0.1 * | |||
|5.381 | |||
|86 | |||
|- | |||
|0.25 | |||
|4.762 | |||
|25.98 | |||
|- | |||
|0.5 | |||
|4.336 | |||
|35.60 | |||
|- | |||
|1 | |||
|4.282 | |||
|43.8 | |||
|- | |||
|2.5 | |||
|3.835 | |||
|93.9 | |||
|- | |||
|5 | |||
|3.704 | |||
|130.5 | |||
|- | |||
|10 | |||
|3.600 | |||
|201.1 | |||
|- | |||
|25 | |||
|3.479 | |||
|383 | |||
|} | |||
[[Image:GlycineCalibration.jpg]] | [[Image:GlycineCalibration.jpg]] |
Revision as of 20:49, 17 September 2014
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September 16, 2014Tasklist1. Prepare 500 mL 50mM NaCl
2. Prepare 1 L 50 mM Glycine Buffer at pH 3.5
3. Prepare 25 mL 1 g/L Lysozyme
Dialysis1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube
2. Dialyze 4 mL of Lysozyme colloid solution in 3500 g/mol MWCO tubing for glycine soak. pH and Conductivity
NOTE The 0.1 mM solution was omitted because all of the data measured were outliers. The omitted data is shown in the table but is NOT included in the graphs.
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