User:Khyra A. Neal/Notebook/Chem 571/2014/09/16: Difference between revisions
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==September 16, 2014== | |||
===Tasklist=== | |||
1. Prepare 500 mL 50mM NaCl | |||
* (50 X 10<sup>-3</sup>M NaCl) * (58.44 g/mol) * (1 L/1000 mL) * 500 mL = 1.461 g NaCl for 500 mL | |||
2. Prepare 1 L 50 mM Glycine Buffer at pH 3.5 | |||
* 50 X 10<sup>-3</sup>M Glycine) * (75.07 g/mol) = 3.754 g in 1 L | |||
*Add 4 mL 1 M HCl to lower pH to 3.5 | |||
3. Prepare 25 mL 1 g/L Lysozyme | |||
*0 .025 g Lysozyme in 25 mL | |||
==Dialysis== | |||
1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube | |||
* 3500 g/mol MWCO tubing in approximately 100 mL of each solution | |||
** NaCl | |||
** Glycine | |||
** Pure Soak Water (HPLC Water) | |||
*25k g/mol MWCO tubing in Glycine soak | |||
2. Dialyze 4 mL of Lysozyme colloid solution in 3500 g/mol MWCO tubing for glycine soak. | |||
==pH and Conductivity== | |||
* Prepare 2x, 5x, 10x, 20x, 50x, 100x, 200x, and 500x dilutions of 50 mM Glycine and NaCl stock solutions. | |||
* The pH and conductivity measurements are summarized in the tables and graphs below. | |||
'''NOTE''' The 0.1 mM solution was omitted because all of the data measured were outliers. The omitted data is shown in the table but is '''NOT''' included in the graphs. | |||
==<font color=#0f1028></font>== | |||
{|style="width:300px" | |||
|<u>NaCl (mM) </u> | |||
|<u>pH</u> | |||
|<u>Conductivity (μS/cm)</u> | |||
|- | |||
|0.1 * | |||
|6.768 | |||
|50.8 | |||
|- | |||
|0.25 | |||
|6.331 | |||
|75.4 | |||
|- | |||
|0.5 | |||
|6.507 | |||
|68.0 | |||
|- | |||
|1 | |||
|6.188 | |||
|290.2 | |||
|- | |||
|2.5 | |||
|5.989 | |||
|304 | |||
|- | |||
|5 | |||
|6.189 | |||
|623 | |||
|- | |||
|10 | |||
|5.963 | |||
|1178 | |||
|- | |||
|25 | |||
|6.064 | |||
|3030 | |||
|} | |||
==<font color=#0f1028></font>== | |||
{|style="width:300px" | |||
|<u>Glycine (mM) </u> | |||
|<u>pH</u> | |||
|<u>Conductivity (μS/cm) </u> | |||
|- | |||
|0.1 * | |||
|5.381 | |||
|86 | |||
|- | |||
|0.25 | |||
|4.762 | |||
|25.98 | |||
|- | |||
|0.5 | |||
|4.336 | |||
|35.60 | |||
|- | |||
|1 | |||
|4.282 | |||
|43.8 | |||
|- | |||
|2.5 | |||
|3.835 | |||
|93.9 | |||
|- | |||
|5 | |||
|3.704 | |||
|130.5 | |||
|- | |||
|10 | |||
|3.600 | |||
|201.1 | |||
|- | |||
|25 | |||
|3.479 | |||
|383 | |||
|} | |||
[[Image:GlycineCalibration.jpg]] | |||
[[Image:GlycineConductivity.jpg]] | |||
[[Image:NaClCalibration.jpg]] | |||
[[Image:NaClConductivity.jpg]] | |||
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September 16, 2014Tasklist1. Prepare 500 mL 50mM NaCl
2. Prepare 1 L 50 mM Glycine Buffer at pH 3.5
3. Prepare 25 mL 1 g/L Lysozyme
Dialysis1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube
2. Dialyze 4 mL of Lysozyme colloid solution in 3500 g/mol MWCO tubing for glycine soak. pH and Conductivity
NOTE The 0.1 mM solution was omitted because all of the data measured were outliers. The omitted data is shown in the table but is NOT included in the graphs.
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