User:Khyra A. Neal/Notebook/Chem 571/2014/09/16: Difference between revisions
From OpenWetWare
(Autocreate 2014/09/16 Entry for User:Khyra_A._Neal/Notebook/Chem_571) |
(fix raw html notebook nav) |
||
(6 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==September 16, 2014== | |||
===Tasklist=== | |||
1. Prepare 500 mL 50mM NaCl | |||
* (50 X 10<sup>-3</sup>M NaCl) * (58.44 g/mol) * (1 L/1000 mL) * 500 mL = 1.461 g NaCl for 500 mL | |||
2. Prepare 1 L 50 mM Glycine Buffer at pH 3.5 | |||
* 50 X 10<sup>-3</sup>M Glycine) * (75.07 g/mol) = 3.754 g in 1 L | |||
*Add 4 mL 1 M HCl to lower pH to 3.5 | |||
3. Prepare 25 mL 1 g/L Lysozyme | |||
*0 .025 g Lysozyme in 25 mL | |||
==Dialysis== | |||
1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube | |||
* 3500 g/mol MWCO tubing in approximately 150 mL of each "soak" solution | |||
** NaCl | |||
** Glycine | |||
** Pure Soak Water (HPLC Water) | |||
*25k g/mol MWCO tubing in Glycine soak | |||
2. Dialyze 4 mL of Lysozyme colloid solution (30:1 [Au]:[Lysozyme]) in 3500 g/mol MWCO tubing for glycine soak. | |||
3. Beakers were labeled, covered with parafilm, and placed on a shaker at low speed overnight. | |||
==pH and Conductivity== | |||
* Prepare 2x, 5x, 10x, 20x, 50x, 100x, 200x, and 500x dilutions of 50 mM Glycine and NaCl stock solutions. | |||
* The pH and conductivity measurements are summarized in the tables and graphs below. | |||
'''NOTE''' The 0.1 mM solution was omitted because all of the data measured were outliers. The omitted data is shown in the table but is '''NOT''' included in the graphs. The outlier is indicated on the table by *. | |||
==<font color=#0f1028></font>== | |||
{|style="width:300px" | |||
|<u>NaCl (mM) </u> | |||
|<u>pH</u> | |||
|<u>Conductivity (μS/cm)</u> | |||
|- | |||
|0.1 * | |||
|6.768 | |||
|50.8 | |||
|- | |||
|0.25 | |||
|6.331 | |||
|75.4 | |||
|- | |||
|0.5 | |||
|6.507 | |||
|68.0 | |||
|- | |||
|1 | |||
|6.188 | |||
|290.2 | |||
|- | |||
|2.5 | |||
|5.989 | |||
|304 | |||
|- | |||
|5 | |||
|6.189 | |||
|623 | |||
|- | |||
|10 | |||
|5.963 | |||
|1178 | |||
|- | |||
|25 | |||
|6.064 | |||
|3030 | |||
|} | |||
==<font color=#0f1028></font>== | |||
{|style="width:300px" | |||
|<u>Glycine (mM) </u> | |||
|<u>pH</u> | |||
|<u>Conductivity (μS/cm) </u> | |||
|- | |||
|0.1 * | |||
|5.381 | |||
|86 | |||
|- | |||
|0.25 | |||
|4.762 | |||
|25.98 | |||
|- | |||
|0.5 | |||
|4.336 | |||
|35.60 | |||
|- | |||
|1 | |||
|4.282 | |||
|43.8 | |||
|- | |||
|2.5 | |||
|3.835 | |||
|93.9 | |||
|- | |||
|5 | |||
|3.704 | |||
|130.5 | |||
|- | |||
|10 | |||
|3.600 | |||
|201.1 | |||
|- | |||
|25 | |||
|3.479 | |||
|383 | |||
|} | |||
[[Image:GlycineCalibration.jpg]] | |||
[[Image:GlycineConductivity.jpg]] | |||
[[Image:NaClCalibration.jpg]] | |||
[[Image:NaClConductivity.jpg]] | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 00:18, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
September 16, 2014Tasklist1. Prepare 500 mL 50mM NaCl
2. Prepare 1 L 50 mM Glycine Buffer at pH 3.5
3. Prepare 25 mL 1 g/L Lysozyme
Dialysis1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube
2. Dialyze 4 mL of Lysozyme colloid solution (30:1 [Au]:[Lysozyme]) in 3500 g/mol MWCO tubing for glycine soak. 3. Beakers were labeled, covered with parafilm, and placed on a shaker at low speed overnight. pH and Conductivity
NOTE The 0.1 mM solution was omitted because all of the data measured were outliers. The omitted data is shown in the table but is NOT included in the graphs. The outlier is indicated on the table by *.
|