User:Khyra A. Neal/Notebook/Chem 571/2014/09/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==September 2, 2014== | ==September 2, 2014== | ||
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Potassium Hydrogen Phthalate (KHPhth) is used a standard for NaOH. | Potassium Hydrogen Phthalate (KHPhth) is used a standard for NaOH. | ||
*Approximately 0.501 g of KHPhth was mixed with 17 mL of water and titrated with NaOH stock solution. Phenolphthalein was used as an indicator and only 5 drops was added. This process was repeated two additional times and approximately the same results were yielded. | *Approximately 0.501 g of KHPhth was mixed with approximately 17 mL of water and titrated with NaOH stock solution. Phenolphthalein was used as an indicator and only 5 drops was added. This process was repeated two additional times and approximately the same results were yielded. | ||
*The final concentration of NaOH was found by averaging the results from the three trials. | *The final concentration of NaOH was found by averaging the results from the three trials. | ||
*Final NaOH concentration is 0.1485 M. | *Final NaOH concentration is 0.1485 M. | ||
'''NOTE''' The final concentration was averaged because the results were similar for all three trials. | '''NOTE''' The final concentration was averaged because the results were similar for all three trials. The amount of water that was added to KHPhth is an estimate because additional water was added to the weigh boat to ensure that all KHPhth measured was used. | ||
==<font color=#0f1028></font>== | ==<font color=#0f1028></font>== | ||
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* 0.3896 g of Isoleucine was dissolved in 10 mL of H<sub>2</sub>O and 10 mL of 21 mM HCl | * 0.3896 g of Isoleucine was dissolved in 10 mL of H<sub>2</sub>O and 10 mL of 21 mM HCl | ||
*The initial pH of the isoleucine solution is 3.351. | *The initial pH of the isoleucine solution is 3.351. | ||
*NaOH was titrated against the isoleucine solution in 2 mL intervals and the pH tested until the pH reached around 10. | *NaOH was titrated against the isoleucine solution in 2 mL intervals and the pH tested until the pH reached around 10. The pH was recorded every 2 mL and a titration curve was created. | ||
'''NOTE''' The pH meter that was used was calibrated using buffers solutions of pH 4,7,and 10. | '''NOTE''' The pH meter that was used was calibrated using buffers solutions of pH 4,7,and 10. |
Latest revision as of 00:13, 27 September 2017
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September 2, 2014Stock Solution Preparations and Titrant StandardizationPreparation of Stock Solution: 0.15 M NaOH was prepared from 3.00g of NaOH and then diluted to 500 mL with H2O.
Tirant Standardization: Potassium Hydrogen Phthalate (KHPhth) is used a standard for NaOH.
NOTE The final concentration was averaged because the results were similar for all three trials. The amount of water that was added to KHPhth is an estimate because additional water was added to the weigh boat to ensure that all KHPhth measured was used.
Isoleucine Titration: HydrophobicUsing the final concentration of 0.1485 M NaOH stock solution, the amount of isoleucine was calculated to yield 20 mL.
NOTE The pH meter that was used was calibrated using buffers solutions of pH 4,7,and 10.
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