User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/24: Difference between revisions
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== | ==Purpose== | ||
* | * DAY 8 - Continue with running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days. | ||
==Procedure== | |||
* The flasks were taken out from orbital shaker. | |||
* 3mL of cell culture were taken from each flasks in a 4mL glass cuvette. | |||
* The absorbance for the 3mL cell culture was taken with wavelength 600.0nm. | |||
* Absorbances were recorded. | |||
==Results== | |||
* A graph of absorbance versus number of days for which the films were soaked in media was plotted. | |||
* The absorbance measured on day 2, 4, 6 and 8 indicates the antibacterial activity exhibited by the media after silver-incorporated films has soaked for 2, 4, 6, or 8 days. All measurements were taken once, thus no error bars are displaced on graph. | |||
[[Image:8-day study on cell growth.png|600px]] | |||
==Observation== | |||
* From the graph, the flasks containing control film and the flasks containing only cells and media all exhibited high absorbance through the 8-day experiment, while the flask containing silver-incorporated film exhibited antibacterial activities for day 2, 4, and 8. | |||
* The absorbance for both control flask and flask containing control films reached between 2.0 to 2.5 after 15-hours of cell growth. This indicates that there were no antibacterial effects within both control flasks. However, the absorbance for flasks containing 33% silver-incorporated film was low for day 2, 4, and 8. The absorbance after 15-hours of cell growth peaked on the 6th day. This showed that there was antibacterial growth in film containing silver. The maximum amount of antibacterial growth is on day 2. | |||
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Revision as of 10:11, 30 April 2013
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Purpose
Procedure
Results
Observation
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