User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/21: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Purpose==
* Insert content here...
* DAY 5 - Continue running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.


==Procedure==
* Three flasks were taken out of the drawer: one flask containing silver-incorporated film, one containing control film, and one containing no film.
* Starter pellet was taken out of freezer and defrosted in room temperature.
* 3mL of LB were added to starter pellet and cells were resuspended.
* 1mL of each resuspended cell culture was added into each flasks.
* All flasks were placed in incubator shaker at 230rpm, 37°C, for 15 hours.
==Observation==
* The flasks containing the control films only appeared cloudy, indicating the flasks were contaminated.


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Latest revision as of 22:40, 26 September 2017

Experimental Biological Chemistry II Main project page
Previous entry      Next entry

Purpose

  • DAY 5 - Continue running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.

Procedure

  • Three flasks were taken out of the drawer: one flask containing silver-incorporated film, one containing control film, and one containing no film.
  • Starter pellet was taken out of freezer and defrosted in room temperature.
  • 3mL of LB were added to starter pellet and cells were resuspended.
  • 1mL of each resuspended cell culture was added into each flasks.
  • All flasks were placed in incubator shaker at 230rpm, 37°C, for 15 hours.

Observation

  • The flasks containing the control films only appeared cloudy, indicating the flasks were contaminated.


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