User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/07

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Purpose

  • Run 15 hour cell growth experiment with silver incorporated clay/polymer film and clay/polymer film made on 2013/04/03

Procedure

  • Starter culture was thawed in room temperature.
  • 3mL of sterile LB media was pipetted into falcon tube to resuspend pellet
  • 1mL of resuspended culture was pipetted into each 50mL sterilized LB.
  • Above two processes were repeated for six more times to make a total of 7 cell cultures in 7 flasks.
  • First flask including LB and cell serve as control.
  • One of each LMT+AG200D film was placed in three other flasks, respectively.
  • One of each AG-incorporated LMT+AG200D film was placed in the last three flasks, respectively.
  • All flasks were placed in incubator shaker for 15 hours, 230rpm, at 37°C.
  • Absorbance of 3mL cell culture was taken every three hours using UV-vis spectrometer at 600.0nm.
  • The absorbance of all cell culture was measured against water.
  • Results were shown in results section.

Results

  • Absorbance of LB is 0.7 at 600nm.
  • See table below for data point collected.
Hour Control (LB+cell) LB+Cell+clay/polymer film #1 LB+Cell+clay/polymer film #2 LB+Cell+clay/polymer film #3 LB+cell+silver incorporated clay/polymer film #1 LB+cell+silver incorporated clay/polymer film #2 LB+cell+silver incorporated clay/polymer film #3
0 0.107 0.107 0.12 0.111 0.116 0.109 0.126
3 0.249 0.143 0.157 0.152 0.133 0.235 0.295
6 1.257 0.786 0.781 0.845 1.34 1.225 0.946
9 1.927 1.854 1.987 1.874 1.972 1.906 2.011
12 2.122 2.131 2.212 2.148 2.101 2.138 2.166
15 2.203 2.314 2.26 2.243 2.178 2.157 2.211
  • Graph below shows the relationship between cell growth and time.



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