User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/07: Difference between revisions
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== | ==Purpose== | ||
* | * Run 15 hour cell growth experiment with silver incorporated clay/polymer film and clay/polymer film made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/03|2013/04/03]] | ||
==Procedure== | |||
* Starter culture was thawed in room temperature. | |||
* 3mL of sterile LB media was pipetted into falcon tube to resuspend pellet | |||
* 1mL of resuspended culture was pipetted into each 50mL sterilized LB. | |||
* Above two processes were repeated for six more times to make a total of 7 cell cultures in 7 flasks. | |||
* First flask including LB and cell serve as control. | |||
* One of each LMT+AG200D film was placed in three other flasks, respectively. | |||
* One of each AG-incorporated LMT+AG200D film was placed in the last three flasks, respectively. | |||
* All flasks were placed in incubator shaker for 15 hours, 230rpm, at 37°C. | |||
* Absorbance of 3mL cell culture was taken every three hours using UV-vis spectrometer at 600.0nm. | |||
* The absorbance of all cell culture was measured against water. | |||
* Results were shown in results section. | |||
==Results== | |||
* Absorbance of LB is 0.7 at 600nm. | |||
* See table below for data point collected. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Hour''' | |||
| align="center" style="background:#f0f0f0;"|'''Control (LB+cell)''' | |||
| align="center" style="background:#f0f0f0;"|'''LB+Cell+clay/polymer film #1''' | |||
| align="center" style="background:#f0f0f0;"|'''LB+Cell+clay/polymer film #2''' | |||
| align="center" style="background:#f0f0f0;"|'''LB+Cell+clay/polymer film #3''' | |||
| align="center" style="background:#f0f0f0;"|'''LB+cell+silver incorporated clay/polymer film #1''' | |||
| align="center" style="background:#f0f0f0;"|'''LB+cell+silver incorporated clay/polymer film #2''' | |||
| align="center" style="background:#f0f0f0;"|'''LB+cell+silver incorporated clay/polymer film #3''' | |||
|- | |||
| 0||0.107||0.107||0.12||0.111||0.116||0.109||0.126 | |||
|- | |||
| 3||0.249||0.143||0.157||0.152||0.133||0.235||0.295 | |||
|- | |||
| 6||1.257||0.786||0.781||0.845||1.34||1.225||0.946 | |||
|- | |||
| 9||1.927||1.854||1.987||1.874||1.972||1.906||2.011 | |||
|- | |||
| 12||2.122||2.131||2.212||2.148||2.101||2.138||2.166 | |||
|- | |||
| 15||2.203||2.314||2.26||2.243||2.178||2.157||2.211 | |||
|} | |||
* Graph below shows the relationship between cell growth and time. | |||
Revision as of 20:47, 7 April 2013
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Purpose
Procedure
Results
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