User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/03

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(Autocreate 2013/04/03 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
Current revision (23:55, 7 April 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Purpose==
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* Insert content here...
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* Prepare films for cell growth study on Sunday.
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* Prepare starter culture.
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==Procedure==
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* AgLMT/polymer and LMT/polymer films were dried over night.
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* From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film.
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* Films were placed in small plastic bag for storage.
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* Cells were harvested from shaker. The cells were placed in centrifuge to spin at 4°C, 4700rpm, for 30 minutes.
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* Supernatents were discarded for each falcon tube.
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* Pellets were placed in -80°C freezer until use.

Current revision

Experimental Biological Chemistry II Main project page
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Purpose

  • Prepare films for cell growth study on Sunday.
  • Prepare starter culture.

Procedure

  • AgLMT/polymer and LMT/polymer films were dried over night.
  • From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film.
  • Films were placed in small plastic bag for storage.
  • Cells were harvested from shaker. The cells were placed in centrifuge to spin at 4°C, 4700rpm, for 30 minutes.
  • Supernatents were discarded for each falcon tube.
  • Pellets were placed in -80°C freezer until use.



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