User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/03: Difference between revisions

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{|{{table}} width="800"
{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Purpose==
==Purpose==
* Prepare films for cell growth study on Sunday.
* Prepare films for cell growth study on Sunday.
* Prepare starter culture.


==Procedure==
==Procedure==
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* From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film.
* From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film.
* Films were placed in small plastic bag for storage.
* Films were placed in small plastic bag for storage.
* Cells were harvested from shaker. The cells were placed in centrifuge to spin at 4°C, 4700rpm, for 30 minutes.
* Supernatents were discarded for each falcon tube.
* Pellets were placed in -80°C freezer until use.




Latest revision as of 22:36, 26 September 2017

Experimental Biological Chemistry II Main project page
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Purpose

  • Prepare films for cell growth study on Sunday.
  • Prepare starter culture.

Procedure

  • AgLMT/polymer and LMT/polymer films were dried over night.
  • From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film.
  • Films were placed in small plastic bag for storage.
  • Cells were harvested from shaker. The cells were placed in centrifuge to spin at 4°C, 4700rpm, for 30 minutes.
  • Supernatents were discarded for each falcon tube.
  • Pellets were placed in -80°C freezer until use.



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