User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/02: Difference between revisions

Latest revision as of 22:35, 26 September 2017

Experimental Biological Chemistry II

The purpose of the experiment today is to make starter culture in order to prepare for a 15-hour cell growth experiment tomorrow.

3mL of starter culture, each in 4 falcon tubes were made.
In 4 falcon tubes labeled "DH5α-T1 starter culture", 3mL of autoclaved LB were pipetted, respectively.
With a wooden stick, a portion of E.coli cells were picked from cell plates made on 02/12/2013 and dipped in one falcon tube containing 3mL of autoclaved LB.
The above procedure was repeated three more times.
Cultures were placed in shaker at 37°C, 230rpm for 2 hours.
After shaking, culture were placed in centrifuge at 4°C, 4700rpm, for 30 minutes.
Supernatants were discarded, and pellet were stored in -80°C until next day morning.

@@ Line 2: / Line 2: @@
 |-
 |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
-|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 |-
 | colspan="2"|