User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/03/05

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(Autocreate 2013/03/05 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
Current revision (10:20, 11 March 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Purpose==
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* Insert content here...
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* Clean up bacteria cells from last week.
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* Autoclave LB for future use.
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==Procedure==
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* Bacteria cells were taken from orbital shaker. In each flask containing cells, 4-5 drops of 1M Wescodyne was added. The flasks were gently swirled to mix.
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* Flasks were left sitting for 4-5 minutes. Liquid in flasks were then poured into Silver Nitrate waste bottle located in Beeghly 207 under fume hood.
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* Flasks were cleaned and dried.
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* 16 Erlenmeyer flasks, each containing 50mL autoclaved LB, were needed for future studies:
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  1 flask for negative control (cells only)
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  1 flask for negative control (none)
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  3 flasks for 50mL AgNO<sub>3</sub> with cells
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  3 flasks for 25mL AgNO<sub>3</sub> with cells
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  3 flasks for 75mL AgNO<sub>3</sub> with cells
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  1 flask for 50mL AgNO<sub>3</sub> alone
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  1 flask for 25mL AgNO<sub>3</sub> alone
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  1 flask for 75mL AgNO<sub>3</sub> alone
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  1 flask for 10mL AgNO<sub>3</sub> alone
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  1 flask for 100mL AgNO<sub>3</sub> alone
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* 12.5g of LB media were weighted out and placed in each 250mL Erlenmeyer flask, and mixed with 50mL sterile water.
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* All flasks were autoclaved under liquid cycle.
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Current revision

Experimental Biological Chemistry Main project page
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Purpose

  • Clean up bacteria cells from last week.
  • Autoclave LB for future use.

Procedure

  • Bacteria cells were taken from orbital shaker. In each flask containing cells, 4-5 drops of 1M Wescodyne was added. The flasks were gently swirled to mix.
  • Flasks were left sitting for 4-5 minutes. Liquid in flasks were then poured into Silver Nitrate waste bottle located in Beeghly 207 under fume hood.
  • Flasks were cleaned and dried.
  • 16 Erlenmeyer flasks, each containing 50mL autoclaved LB, were needed for future studies:
  1 flask for negative control (cells only)
  1 flask for negative control (none)
  3 flasks for 50mL AgNO3 with cells
  3 flasks for 25mL AgNO3 with cells
  3 flasks for 75mL AgNO3 with cells
  1 flask for 50mL AgNO3 alone
  1 flask for 25mL AgNO3 alone
  1 flask for 75mL AgNO3 alone
  1 flask for 10mL AgNO3 alone
  1 flask for 100mL AgNO3 alone
  • 12.5g of LB media were weighted out and placed in each 250mL Erlenmeyer flask, and mixed with 50mL sterile water.
  • All flasks were autoclaved under liquid cycle.


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