User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/14
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< User:Keyun Wang | Notebook | Experimental Biological Chemistry I | 2013 | 02(Difference between revisions)
(Autocreate 2013/02/14 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I) |
Current revision (19:42, 18 February 2013) (view source) |
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| - | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | + | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span> |
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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| - | == | + | ==Purpose== |
| - | * | + | * To harvest cells grown on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/13|2013/02/13]] and store pellet in -80°C freezer for future use. |
| + | |||
| + | ==Procedure== | ||
| + | * Cells were taken off the orbital shaker and were centrifuged at 4700rpm, at 4°C, for 30 minutes. | ||
| + | * The supernatent was poured out, leaving the pellet alone. | ||
| + | * The pellet was placed in -80°C freezer. | ||
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