User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/13

From OpenWetWare

< User:Keyun Wang | Notebook | Experimental Biological Chemistry I | 2013 | 02(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/02/13 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
Current revision (12:50, 13 February 2013) (view source)
 
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Purpose==
-
* Insert content here...
+
* Make starter culture of DH5α-T1 cells in order to start pilot study.
 +
 
 +
==Procedure==
 +
* DH5α-T1 plate was taken out this morning and placed in 20°C refrigerator
 +
* In a sterile environment, 3mL of autoclaved LB was transferred into a 15mL falcon tube.
 +
* With an autoclaved wooden stick, a cell colony from cell plate was obtained and dipped into the 3mL LB.
 +
* The cell containing LB media was then placed into an orbital shaker at 230rpm for 16 hours.

Current revision

Experimental Biological Chemistry II Main project page
Previous entry      Next entry

Purpose

  • Make starter culture of DH5α-T1 cells in order to start pilot study.

Procedure

  • DH5α-T1 plate was taken out this morning and placed in 20°C refrigerator
  • In a sterile environment, 3mL of autoclaved LB was transferred into a 15mL falcon tube.
  • With an autoclaved wooden stick, a cell colony from cell plate was obtained and dipped into the 3mL LB.
  • The cell containing LB media was then placed into an orbital shaker at 230rpm for 16 hours.



Personal tools