User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/13

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Experimental Biological Chemistry II

DH5α-T1 plate was taken out this morning and placed in 20°C refrigerator
In a sterile environment, 3mL of autoclaved LB was transferred into a 15mL falcon tube.
With an autoclaved wooden stick, a cell colony from cell plate was obtained and dipped into the 3mL LB.
The cell containing LB media was then placed into an orbital shaker at 230rpm for 16 hours.

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-|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Experimental Biological Chemistry II</span>
 |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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-==Entry title==
+==Purpose==
-* Insert content here...
+* Make starter culture of DH5α-T1 cells in order to start pilot study.
+==Procedure==
+* DH5α-T1 plate was taken out this morning and placed in 20°C refrigerator
+* In a sterile environment, 3mL of autoclaved LB was transferred into a 15mL falcon tube.
+* With an autoclaved wooden stick, a cell colony from cell plate was obtained and dipped into the 3mL LB.
+* The cell containing LB media was then placed into an orbital shaker at 230rpm for 16 hours.