User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12

From OpenWetWare
Jump to navigationJump to search
Experimental Biological Chemistry II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • Start DH5α-T1 cell growth on petri dish for future use.

Procedure

  • Plates plated from 2013/02/06 were transferred to fridge at 20°C due to neglect.
  • In order to ensure consistent cell growth for each experiment, a new dish was plated.
  • In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on 2013/02/06 and strikes on a new LB+agar plate.
  • The dish was placed in -37°C for over night (16 hours).


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/02/12&oldid=676063"