User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12

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(Autocreate 2013/02/12 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
Current revision (13:44, 13 February 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Purpose==
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* Insert content here...
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* Start DH5α-T1 cell growth on petri dish for future use.
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==Procedure==
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* Plates plated from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06|2013/02/06]] were transferred to fridge at 20°C due to neglect.
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* In order to ensure consistent cell growth for each experiment, a new dish was plated.
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* In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on  [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06|2013/02/06]] and strikes on a new LB+agar plate.
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* The dish was placed in -37°C for over night (16 hours).
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Current revision

Experimental Biological Chemistry II Main project page
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Purpose

  • Start DH5α-T1 cell growth on petri dish for future use.

Procedure

  • Plates plated from 2013/02/06 were transferred to fridge at 20°C due to neglect.
  • In order to ensure consistent cell growth for each experiment, a new dish was plated.
  • In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on 2013/02/06 and strikes on a new LB+agar plate.
  • The dish was placed in -37°C for over night (16 hours).


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