User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/02/12 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
 
No edit summary
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Purpose==
* Insert content here...
* Start DH5α-T1 cell growth on petri dish for future use.


==Procedure==
* Plates plated from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06|2013/02/06]] were transferred to fridge at 20°C due to neglect.
* In order to ensure consistent cell growth for each experiment, a new dish was plated.
* In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on  [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06|2013/02/06]] and strikes on a new LB+agar plate.
* The dish was placed in -37°C for over night (16 hours).


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 10:44, 13 February 2013

Experimental Biological Chemistry II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • Start DH5α-T1 cell growth on petri dish for future use.

Procedure

  • Plates plated from 2013/02/06 were transferred to fridge at 20°C due to neglect.
  • In order to ensure consistent cell growth for each experiment, a new dish was plated.
  • In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on 2013/02/06 and strikes on a new LB+agar plate.
  • The dish was placed in -37°C for over night (16 hours).


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2013/02/12&oldid=676063"