User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Purpose== | ||
* | * Start DH5α-T1 cell growth on petri dish for future use. | ||
==Procedure== | |||
* Plates plated from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06|2013/02/06]] were transferred to fridge at 20°C due to neglect. | |||
* In order to ensure consistent cell growth for each experiment, a new dish was plated. | |||
* In a sterile environment, an autoclaved wooden stick was used to dip into the DH5α-T1 disk made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/06|2013/02/06]] and strikes on a new LB+agar plate. | |||
* The dish was placed in -37°C for over night (16 hours). | |||
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Latest revision as of 22:27, 26 September 2017
Experimental Biological Chemistry II | Main project page Previous entry Next entry |
Purpose
Procedure
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