User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Purpose== | ||
* | * Grind Ag+ Clay into powder for X-ray spectroscopic analysis. | ||
* Grow DH5α-T1 bacteria cells and make two glycerol stocks of it. | |||
==Procedure== | |||
* Procedure for grinding of Ag+ Clay into powder form is recorded in [[User:Melissa Novy/Notebook/CHEM-572/2013/02/05|Melissa's Notebook]]. | |||
* Growing of DH5α-T1 cells | |||
** One shot of DH5α-T1 cells was taken out from -87°C fridge. | |||
** Cells were thawed in room temperature | |||
** 3mL of autoclaved LB media was pipetted into a test tube | |||
** Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media. | |||
** The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm. | |||
** After two hours, the culture appeared clear, indicating no cell growth, an alternative approach were taken. | |||
* A different cell line was used for cell amplification, and the procedure for DH5α-T1 cell amplification was altered then repeated. | |||
** DH10B cells were taken from -87°C fridge. | |||
** The cell were let thaw on ice. | |||
** In two clean 15mL falcon tubes, 3mL autoclaved LB media were added. | |||
** In one falcon tube, 10uL DH10B cells were added. | |||
** In another falcon tube, 10uL DH5α-T1 cells were added. | |||
** The two falcon tubes were placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm. | |||
** After 2 hours of incubation, the both cultures appeared clear. As a result, both cultures were placed in incubator horizontally to maximize shaking and left in shaker under same conditions for over night. | |||
Revision as of 17:21, 5 February 2013
Experimental Biological Chemistry II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Purpose
Procedure
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