User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/05

Purpose

• Grind Ag+ Clay into powder for X-ray spectroscopic analysis.
• Grow DH5α-T1 bacteria cells and make two glycerol stocks of it.

Procedure

• Procedure for grinding of Ag+ Clay into powder form is recorded in Melissa's Notebook.
• Growing of DH5α-T1 cells
  • One shot of DH5α-T1 cells was taken out from -87°C fridge.
  • Cells were thawed in room temperature
  • 3mL of autoclaved LB media was pipetted into a test tube
  • Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media.
  • The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.