User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/01/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Purpose== | ||
* | * The purpose today is to autoclave LB medium to prepare for cell culture. | ||
* Also, Ag+ incorporated clay is going to be filtered with water and ethanol to isolate clay from water/ethanol solution. | |||
==Procedure== | |||
* 50mL LB media was made using the following way: | |||
0.50g Bacto-Trypsin | |||
0.25g Bacto-Yeast Extract | |||
0.50g Sodium Chloride | |||
50mL of distilled water | |||
Note: LB media obtained from Genova® with 2:1:2 proportion was used instead. 1.25g of LB powder was weighted out. | |||
* Each of the 50mL LB media was placed in two 125mL glass bottles and four 250mL Erlenmeyer flask. | |||
* 500mL of Agar was made using the following in a 1000mL Erlenmeyer flask: | |||
12.5g of LB media powder | |||
500mL of distilled water | |||
7.5g of Trypsin Soy Agar | |||
* The Erlenmeyer flask obtaining LB media or Agar were covered with aluminum foil, the bottles containing LB were capped loosely. All media were autoclaved under liquid cycle. | |||
* The clay+AgNO<sub>3</sub> mixture was taken off hot plate. | |||
* A filter funnel, attached to 500mL Erlenmeyer flask then attached to a pump, is covered with a paper filter. Surface of paper filter was wetted and attached onto filter funnel through adding water. | |||
* The clay+AgNO<sub>3</sub> mixture was then transfered into the filter funnel with suction applied. | |||
* Aluminum foil was used to cover the funnel to avoid silver to interact with light. | |||
==Results== | |||
* The clay+AgNO<sub>3</sub> mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color. | |||
Revision as of 12:00, 30 January 2013
Experimental Biological Chemistry II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Purpose
Procedure
0.50g Bacto-Trypsin 0.25g Bacto-Yeast Extract 0.50g Sodium Chloride 50mL of distilled water Note: LB media obtained from Genova® with 2:1:2 proportion was used instead. 1.25g of LB powder was weighted out.
12.5g of LB media powder 500mL of distilled water 7.5g of Trypsin Soy Agar
Results
|