User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/01/30: Difference between revisions
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* The clay+AgNO<sub>3</sub> mixture was then transfered into the filter funnel with suction applied. | * The clay+AgNO<sub>3</sub> mixture was then transfered into the filter funnel with suction applied. | ||
* Aluminum foil was used to cover the funnel to avoid silver to interact with light. | * Aluminum foil was used to cover the funnel to avoid silver to interact with light. | ||
* After autoclave, the LB media were placed on counter without removing the aluminum foil. The Agar were cooled to about 50°C, until the wall of Erlenmeyer flask can be touched by hand. Approximately 20mL of the Agar were poured into each of the 20 separate petri dishes. | |||
==Results== | ==Results== | ||
* The clay+AgNO<sub>3</sub> mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color. | * The clay+AgNO<sub>3</sub> mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color. However, the filtered liquid solution turned dark and obtained a darker red color after sitting on the counter. The filtered liquid solution was analyzed by Dr. Fox, who later concluded that silver nanoparticles were found in filtrate. | ||
Revision as of 10:30, 1 February 2013
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Purpose
Procedure
0.50g Bacto-Trypsin 0.25g Bacto-Yeast Extract 0.50g Sodium Chloride 50mL of distilled water Note: LB media obtained from Genova® with 2:1:2 proportion was used instead. 1.25g of LB powder was weighted out.
12.5g of LB media powder 500mL of distilled water 7.5g of Trypsin Soy Agar
Results
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