User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/01/30

From OpenWetWare

< User:Keyun Wang | Notebook | Experimental Biological Chemistry I | 2013 | 01(Difference between revisions)
Jump to: navigation, search
Current revision (12:30, 1 February 2013) (view source)
 
Line 27: Line 27:
* The clay+AgNO<sub>3</sub> mixture was then transfered into the filter funnel with suction applied.
* The clay+AgNO<sub>3</sub> mixture was then transfered into the filter funnel with suction applied.
* Aluminum foil was used to cover the funnel to avoid silver to interact with light.
* Aluminum foil was used to cover the funnel to avoid silver to interact with light.
 +
* After autoclave, the LB media were placed on counter without removing the aluminum foil. The Agar were cooled to about 50°C, until the wall of Erlenmeyer flask can be touched by hand. Approximately 20mL of the Agar were poured into each of the 20 separate petri dishes.
==Results==
==Results==
-
* The clay+AgNO<sub>3</sub> mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color.
+
* The clay+AgNO<sub>3</sub> mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color. However, the filtered liquid solution turned dark and obtained a darker red color after sitting on the counter. The filtered liquid solution was analyzed by Dr. Fox, who later concluded that silver nanoparticles were found in filtrate.

Current revision

Experimental Biological Chemistry II Main project page
Previous entry      Next entry

Purpose

  • The purpose today is to autoclave LB medium to prepare for cell culture.
  • Also, Ag+ incorporated clay is going to be filtered with water and ethanol to isolate clay from water/ethanol solution.

Procedure

  • 50mL LB media was made using the following way:
  0.50g Bacto-Trypsin
  0.25g Bacto-Yeast Extract
  0.50g Sodium Chloride
  50mL of distilled water
  Note: LB media obtained from Genova® with 2:1:2 proportion was used instead. 1.25g of LB powder was weighted out.
  • Each of the 50mL LB media was placed in two 125mL glass bottles and four 250mL Erlenmeyer flask.
  • 500mL of Agar was made using the following in a 1000mL Erlenmeyer flask:
  12.5g of LB media powder
  500mL of distilled water
  7.5g of Trypsin Soy Agar
  • The Erlenmeyer flask obtaining LB media or Agar were covered with aluminum foil, the bottles containing LB were capped loosely. All media were autoclaved under liquid cycle.
  • The clay+AgNO3 mixture was taken off hot plate.
  • A filter funnel, attached to 500mL Erlenmeyer flask then attached to a pump, is covered with a paper filter. Surface of paper filter was wetted and attached onto filter funnel through adding water.
  • The clay+AgNO3 mixture was then transfered into the filter funnel with suction applied.
  • Aluminum foil was used to cover the funnel to avoid silver to interact with light.
  • After autoclave, the LB media were placed on counter without removing the aluminum foil. The Agar were cooled to about 50°C, until the wall of Erlenmeyer flask can be touched by hand. Approximately 20mL of the Agar were poured into each of the 20 separate petri dishes.

Results

  • The clay+AgNO3 mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color. However, the filtered liquid solution turned dark and obtained a darker red color after sitting on the counter. The filtered liquid solution was analyzed by Dr. Fox, who later concluded that silver nanoparticles were found in filtrate.



Personal tools