User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/28

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Purpose

  • Au/ADA samples made on 2012/11/27 were ran on UV-vis spectrophotometer and Atomic Absorption Spectrometer, results were analyzed.
  • Au/ADA samples were resuspended in a range of Tris buffers to test resuspension based on concentration of buffer and pH.

Procedure for Running Au/ADA samples on UV-vis Spectrophotometer

  • 3mL of Au/ADA samples with the following mole ratios were analyzed using UV-vis spectrophotometer.
  60-70-80-90-100-110-120-130-140-150
  • 3mL of distilled water was run on UV-vis under spectrum method. The absorbance obtained from wavelength range 200nm to 800nm were used as base line correction for the Au/ADA samples.

Results for Running Au/ADA samples on UV-vis Spectrophotometer

Procedure for Running Au/ADA samples on Atomic Absorption Spectrometer

Results for Running Au/ADA samples on Atomic Absorption Spectrometer

Procedure for Au/ADA Resuspension in Tris Buffer

Results for Au/ADA Resuspension in Tris Buffer


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