User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/28: Difference between revisions
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==Results for Au/ADA Resuspension in Tris Buffer== | ==Results for Au/ADA Resuspension in Tris Buffer== | ||
* All solutions were successfully resuspended after the addition of Tris buffer. The solution after resuspension appeared as purple homogenous mixture and are clear in color. A picture taken after resuspension of all resuspended Au/ADA samples is shown below: | |||
[[Image:Au ADA after resuspension.jpg|600px]] | |||
* The color for solution for 110 Au/ADA were clearer than other Au/ADA samples because the solution was lighter in color before resuspension. | |||
* The amount of time it took for each solution to resuspend was recorded in table below: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Mole ratio of Au/ADA''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Tris buffer used[uM]''' | |||
| align="center" style="background:#f0f0f0;"|'''pH of Tris buffer''' | |||
| align="center" style="background:#f0f0f0;"|'''Time for resuspension[sec]''' | |||
|- | |||
| 60||1||10||3 | |||
|- | |||
| 70||10||10||4 | |||
|- | |||
| 80||100||10||4 | |||
|- | |||
| 90||1||8||7 | |||
|- | |||
| 100||10||8||6 | |||
|- | |||
| 110||100||8||6 | |||
|} | |||
* From table above, it can be concluded that resuspension happened faster in solutions with Tris buffer pH 10.0 then solutions with Tris buffer pH 8.0. | |||
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Revision as of 21:27, 30 November 2012
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Purpose
Procedure for Running Au/ADA samples on UV-vis Spectrophotometer
60-70-80-90-100-110-120-130-140-150
Results for Running Au/ADA samples on UV-vis Spectrophotometer
Procedure for Running Au/ADA samples on Atomic Absorption Spectrometer
5-8-10-15-20-25-30-40
Results for Running Au/ADA samples on Atomic Absorption Spectrometer
Absorbance = 0.0153 * Concentration + 0.045
Procedure for Au/ADA Resuspension in Tris Buffer
Results for Au/ADA Resuspension in Tris Buffer
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