User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/28: Difference between revisions
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[[Image:Water and Au ADA.jpg|350px]] | [[Image:Water and Au ADA.jpg|350px]] | ||
* From the graph above, it can be concluded that there was no significant differences between the absorbance between water samples and each Au/ADA samples due to low standard deviation. This indicates that there is no relationship between the mole ratios of Au/ADA in solution and the gold nanoparticle formation in solution. | * From the graph above, it can be concluded that there was no significant differences between the absorbance between water samples and each Au/ADA samples due to low standard deviation. This indicates that there is no relationship between the mole ratios of Au/ADA in solution and the gold nanoparticle formation in solution. | ||
* The data can be compared against the Au/ADA solution made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/27|2012/11/27]]. | * The data can be compared against the Au/ADA solution made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/27|2012/11/27]]. While the Au/ADA before dialysis appeared to have influence between the mole ratio of gold to ADA and concentration of gold nanoparticles in solution, the Au/ADA samples after dialysis does not. This can be explained by the presence of salt in solution that encouraged gold nanoparticle formation but can stabilize the protein nanoparticles to prevent nanoparticles from aggregating. | ||
* Furthermore, the solutions for Au/ADA samples before dialysis did not appear purple, while solutions for Au/ADA samples after dialysis formed purple solutions and purple fibers. This can also be explained by the presence of salt in solution. The presence of salt might interact with gold nanoparticles in a way that encouraged gold nanoparticle configuration that does not absorbce at 525nm. The absence of salt in solution might allow gold nanoparticle to adapt another confirmation that does absorb at 525nm. | |||
==Procedure for Running Au/ADA samples on Atomic Absorption Spectrometer== | ==Procedure for Running Au/ADA samples on Atomic Absorption Spectrometer== | ||
* The same sample of Au/ADA were run on Atomic Absorption Spectrometer. | |||
* Atomic Absorption Spectrometer was calibrated by running HCl with gold at the following parts per million: | |||
5-8-10-15-20-25-30-40 | |||
* HCl was ran to for blank, and water was ran to set base line. | |||
* Au/ADA samples were run, and the sample tubing was raised with distilled water after each sample. | |||
==Results for Running Au/ADA samples on Atomic Absorption Spectrometer== | ==Results for Running Au/ADA samples on Atomic Absorption Spectrometer== | ||
* The HCl with gold for calibration made up a calibration curve for establishing the concentration of Au/ADA samples. The absorbance measured for HCl with gold were listed in the following table: | |||
==Procedure for Au/ADA Resuspension in Tris Buffer== | ==Procedure for Au/ADA Resuspension in Tris Buffer== | ||
==Results for Au/ADA Resuspension in Tris Buffer== | ==Results for Au/ADA Resuspension in Tris Buffer== |
Revision as of 16:59, 30 November 2012
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Purpose
Procedure for Running Au/ADA samples on UV-vis Spectrophotometer
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Results for Running Au/ADA samples on UV-vis Spectrophotometer
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Procedure for Running Au/ADA samples on Atomic Absorption Spectrometer
5-8-10-15-20-25-30-40
Results for Running Au/ADA samples on Atomic Absorption Spectrometer
Procedure for Au/ADA Resuspension in Tris BufferResults for Au/ADA Resuspension in Tris Buffer |