User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/27: Difference between revisions
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* Both Au/HRP and Au/ADA solution exhibit positive linear relationship between increased mole ratio and concentration of gold in solution. This suggested that both enzyme undergoes similar mechanism when interacting with HAuCl<sub>4</sub> under heat. | * Both Au/HRP and Au/ADA solution exhibit positive linear relationship between increased mole ratio and concentration of gold in solution. This suggested that both enzyme undergoes similar mechanism when interacting with HAuCl<sub>4</sub> under heat. | ||
==Procedure for Fiber resuspension in Tris buffer== | |||
* Au/HRP samples with mole ratio 130 to 150 were used to test fiber resuspension with Tris buffer. | |||
* Tris buffer with the following concentrations were used for test resuspension: | |||
** 10mM Tris buffer at pH 10.0 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05|2012/09/05]] | |||
** 50mM Tris buffer at pH 10.0 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/12|2012/09/12]] | |||
** 500mM Tris buffer at pH 10.0 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/12|2012/09/12]] | |||
* A table was made to list the Tris buffer used for resuspension in Au/HRP samples: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Tris Buffer used''' | |||
| align="center" style="background:#f0f0f0;"|'''Au/HRP samples used''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of Buffer added''' | |||
|- | |||
| 10mM at pH 10||130 Au/HRP||1mL | |||
|- | |||
| 50mM at pH 10||140 Au/HRP||1mL | |||
|- | |||
| 500mM at pH 10||150 Au/HRP||1mL | |||
|} | |||
* After resuspension, the solution with added buffer was left in room temperature. The resuspension was checked after 24 hours for possibilities of fiber precipitation. | |||
==Results for Fiber resuspension in Tris buffer== | |||
* The fibers were resuspended fully after pipetting solution up and down to mix. A picture of resuspended solution is shown below: | |||
[[ | |||
* After 24 hours, no purple fibers were formed, thus it can be concluded that the addition of salt into solution interacts with gold nanoparticle aggregation in a way that disrupts the aggregation. The lack of precipitation after 24 hours can be due to the gold nanoparticles being more stable when interacting with Tris buffer. | |||
* A purple homogenous solution was yielded after the addition of Tris buffers at all three concentrations used. Thus, it can be safe to conclude that Tris buffer at pH 10.0 can successfully resuspend fibers at a 6:1 ratio of solution to tris buffer. | |||
* Although the exact mechanism for resuspension is unknown, successful resuspension might be explained with following reasoning: | |||
** | |||
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Revision as of 13:16, 28 November 2012
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Purpose
Procedure for Dialysis
Notes for Dialysis
Procedure for making dialyzed Au/ADA samples
60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150
Procedure for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Results for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Procedure for Running AA Spectrometer on Au/ADA and Au/HRP samples
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Results for Running AA Spectrometer on Au/ADA and Au/HRP samples
Procedure for Fiber resuspension in Tris buffer
Results for Fiber resuspension in Tris buffer
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