User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/27: Difference between revisions
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* From the graph above, it can be concluded that as the mole ratio of Au to ADA increases, the concentration of gold in solution also increases. However, in the above data, there was an outlier at 100 Au/ADA with the calculated concentration 5.2261ppm. This outlier might be due to a lack of raising before sample 100 Au/ADA was injected, leaving more gold residue to be analyzed compare to before. | * From the graph above, it can be concluded that as the mole ratio of Au to ADA increases, the concentration of gold in solution also increases. However, in the above data, there was an outlier at 100 Au/ADA with the calculated concentration 5.2261ppm. This outlier might be due to a lack of raising before sample 100 Au/ADA was injected, leaving more gold residue to be analyzed compare to before. | ||
* It can be seen that the concentration of gold in solution in Au/HRP appeared almost double as the concentration of gold in Au/ADA solutions. This result could be explained with following reasons: | * It can be seen that the concentration of gold in solution in Au/HRP appeared almost double as the concentration of gold in Au/ADA solutions. This result could be explained with following reasons: | ||
** The HRP is more flexible in structure and thus can lead to more HRP folding within 85°C and 4 hours of heating. The ADA might have a less flexible structure, and might be limited with the presence of more beta sheets | ** The HRP is more flexible in structure and thus can lead to more HRP folding within 85°C and 4 hours of heating. The ADA might have a less flexible structure, and might be limited with the presence of more beta sheets within the protein structure. | ||
** The HRP | |||
Revision as of 12:35, 28 November 2012
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Purpose
Procedure for Dialysis
Notes for Dialysis
Procedure for making dialyzed Au/ADA samples
60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150
Procedure for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Results for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Procedure for Running AA Spectrometer on Au/ADA and Au/HRP samples
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Results for Running AA Spectrometer on Au/ADA and Au/HRP samples
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